Anti-SLC25A26 Rabbit Polyclonal Antibody for WB and ELISA - Q70HW3

Core Product Specifications and Parameters

Biological Background: SLC25A26 Function and Localization

• Mitochondrial S-adenosylmethionine carrier protein (SLC25A26) mediates the antiport of cytosolic S-adenosyl-L-methionine (SAM) into the mitochondrial matrix in exchange for S-adenosylhomocysteine (SAH), a by-product of methylation reactions.
• This carrier supplies SAM, the major methyl-group donor, for essential mitochondrial methylation processes, including those affecting DNA, RNA, proteins, and lipids.
• SLC25A26 belongs to the solute carrier family 25 of mitochondrial carriers, characterized by internal sequence repeats and six transmembrane helices.
• It is an integral multipass membrane protein localized to the mitochondrial inner membrane.
• Widely expressed, with highest levels in testis, and moderate to low expression in brain, heart, kidney, lung, skeletal muscle, pancreas, small intestine, and liver.
• Alternative splicing produces multiple transcript variants; mutations in the SLC25A26 gene are associated with mitochondrial disease (COXPD28).
• Keywords relevant to this protein include antiport, disease variant, primary mitochondrial disease, and S-adenosyl-L-methionine binding.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 105-185, a region likely exposed to the matrix side of the inner membrane; this design may favor recognition of the native protein.
• For Western blot, consider using a mitochondrial-enriched lysate or a positive control tissue such as testis, and optimize reducing/denaturing conditions to detect the ~29 kDa band.
• When performing ELISA, titrate the antibody to determine optimal coating concentrations and include appropriate controls (e.g., recombinant protein or peptide immunogen) to assess specificity.
• As a polyclonal antibody, performance may vary between lots; it is advisable to validate each new lot in the intended assay system.
• Cross-reactivity with mouse expands utility for orthologous studies, but always confirm specificity in the relevant species context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, laboratories, and research institutes by providing one-stop access to IVD raw materials, technical services, and consulting from concept to clinic. This SLC25A26 antibody is part of our mitochondrial transporter portfolio, backed by our ability to supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your assay development.