Anti-SLC25A24 Polyclonal Antibody for WB, IF/ICC, ELISA - Q6NUK1

Core Product Specifications and Parameters

Biological Background: SLC25A24 Function and Localization

• SLC25A24 encodes the mitochondrial adenyl nucleotide antiporter SLC25A24, also known as SCaMC-1, APC1, or solute carrier family 25 member 24.
• Acts as an electroneutral antiporter that mediates transport of adenyl nucleotides through the inner mitochondrial membrane; originally identified as an ATP-Mg/Pi antiporter but with broad specificity for adenyl nucleotides. Related references: PMID:15123600 PMID:22015608
• Regulates the mitochondrial matrix adenyl nucleotide pool, adapting to cellular energetic demands and indirectly modulating nucleotide-dependent metabolic pathways. Related references: PMID:15123600 PMID:22015608
• In vitro, exhibits low activity with guanyl and pyrimidine nucleotides. Related references: PMID:15123600
• May protect cells against oxidative stress-induced cell death by buffering mitochondrial calcium levels via formation of calcium-phosphate precipitates. Related references: PMID:22015608 PMID:29100093
• Localizes to the mitochondrial inner membrane.
• Expressed ubiquitously, with highest levels in testis, intermediate in small intestine and pancreas, and weak in kidney, spleen, liver, skeletal muscle, and heart.
• Features alternative splicing and acetylation; contains mitochondrial carrier domains and transmembrane helices; a 3D structure is available.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1–200 (N-terminal region) of human SLC25A24; the antibody may recognize this epitope region. Consider using the recombinant immunogen as a positive control in ELISA.
• For Western blotting (WB), a band around 53 kDa is expected. Validate with mitochondrial-enriched fractions or lysates from high-expression tissues (e.g., testis).
• In immunofluorescence/immunocytochemistry (IF/ICC), colocalization with mitochondrial markers is recommended to confirm specificity. Cell permeabilization and fixation conditions should be optimized for mitochondrial antigens.
• This polyclonal antibody has reported cross-reactivity with human, mouse, and rat SLC25A24; validate reactivity in the species and sample system relevant to your experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutions with a comprehensive source for IVD raw materials, from concept to clinic. For mitochondrial transport and calcium homeostasis research, we offer validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Contact us to streamline your procurement of high-quality SLC25A24 antibodies and related supplies.