Anti-SLC25A23 Polyclonal Antibody for WB, IF/ICC, ELISA - Q9BV35

Core Product Specifications and Parameters

Biological Background: SLC25A23 Function and Localization

• SLC25A23, also known as mitochondrial adenyl nucleotide antiporter SLC25A23 or SCaMC-3, is a member of the solute carrier family 25 and functions as an electroneutral antiporter in the inner mitochondrial membrane.
• It mediates the transport of adenine nucleotides, originally identified as an ATP-magnesium/inorganic phosphate antiporter but also acting as a broad specificity adenyl nucleotide antiporter, thereby regulating the mitochondrial matrix adenine nucleotide pool to adapt to changing cellular energetic demands. Related references: PMID:15123600
• SLC25A23 also serves as a regulator of mitochondrial calcium uptake and can transport trace amounts of other divalent metal cations in complex with ATP. Related references: PMID:24430870, PMID:28695448
• In vitro, a low activity is observed with guanyl and pyrimidine nucleotides. Related references: PMID:15123600
• The protein is localized to the mitochondrial inner membrane.
• Expression is ubiquitous but highest in brain, skeletal muscle, and pancreas.
• It contains transmembrane helices and calcium-binding EF-hand like domains, and undergoes alternative splicing to produce multiple isoforms.

Experimental Guidance and Technical Tips

• For Western blotting, endogenous SLC25A23 is expected at approximately 52 kDa. Consider using lysates from brain, skeletal muscle, or pancreas as positive controls.
• In immunofluorescence/ICC, optimize fixation and permeabilization protocols to detect mitochondrial localization. Co-staining with a mitochondrial marker is recommended.
• The antibody is suitable for ELISA; validate the assay conditions with relevant recombinant protein or peptide standards.
• As a polyclonal antibody, batch-to-batch consistency should be verified, and pre-adsorption controls may be useful for confirming specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with a streamlined sourcing channel for IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies, and ancillary reagents. For mitochondrial transport research, we offer high-quality anti-SLC25A23 antibodies along with custom sourcing of rare targets to support your assay development pipeline.