Anti-SLC25A21 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9BQT8

Core Product Specifications and Parameters

Biological Background: SLC25A21 Function and Localization

• SLC25A21 encodes the mitochondrial 2-oxodicarboxylate carrier, also known as 2-oxoadipate carrier, a member of the solute carrier family 25.
• This carrier transports dicarboxylates across the inner mitochondrial membrane via a counter-exchange mechanism, with substrates including 2-oxoadipate, 2-oxoglutarate, adipate, glutarate, and to a lesser extent, pimelate, 2-oxopimelate, 2-aminoadipate, oxaloacetate, and citrate. Related references: PMID:11083877
• It plays a central role in the catabolism of lysine, hydroxylysine, and tryptophan by importing common metabolite intermediates (e.g., 2-oxoadipate) into mitochondria for conversion to acetyl-CoA and entry into the TCA cycle.
• The protein is localized to the mitochondrial inner membrane and belongs to the mitochondrial carrier repeat-containing protein family with transmembrane helices.
• Alternative splicing produces multiple isoforms; the canonical isoform has a predicted molecular weight of 33 kDa.
• Tissue expression includes placenta, gall bladder, and colon; mutations are associated with primary mitochondrial disease (MTDPS18).
• It functions as an antiporter and is involved in lipid transport; annotated with keywords including Alternative splicing, Antiport, Disease variant, and Primary mitochondrial disease.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 148–247 of human SLC25A21 (NP_085134.1). For applications like IHC-P, consider using antigen retrieval methods optimized for mitochondrial membrane proteins to improve epitope accessibility.
• Predicted molecular weight is 33 kDa; in Western blot, verify band size relative to appropriate molecular weight markers and consider potential post-translational modifications or isoforms that may cause slight shifts.
• This antibody is reported to work in WB, IHC-P, and ELISA. For IHC-P, validate staining specificity using appropriate positive (e.g., placenta, colon) and negative control tissues, and confirm mitochondrial localization.
• If cross-reacting with rat samples, ensure sequence homology of the immunogen region is sufficient to maintain reactivity.
• Recombinant protein or peptide blocking experiments can be used to confirm antibody specificity in functional assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing, supporting diagnostic manufacturers and research labs from concept to clinic. For studies of mitochondrial transporter trafficking and metabolic disorders, our dedicated supply chain can promptly deliver the necessary immunological reagents to accelerate your assay development and biomarker research.