Anti-SLC25A20 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O43772

Core Product Specifications and Parameters

Biological Background: SLC25A20 Function and Localization

• SLC25A20 (CAC/CACT) is a mitochondrial carnitine/acylcarnitine carrier protein belonging to the solute carrier family 25, primarily responsible for the transport of fatty acylcarnitines into mitochondria for beta-oxidation.
• It facilitates the electroneutral antiport of acylcarnitines (ranging from short-chain to long-chain) with free carnitine across the inner mitochondrial membrane, an essential step in energy production from fatty acids. Related references: PMID:12892634, PMID:18307102
• Additionally, it can mediate the uniport of carnitine at lower rates, demonstrating functional versatility in substrate translocation. Related references: PMID:18307102
• The protein is localized exclusively to the mitochondrial inner membrane, consistent with its role in metabolite shuttling.
• Post-translational acetylation (UniProt keyword) may regulate its activity or stability, although the specific sites and functional impact remain to be fully characterized.
• The UniProt entry annotates SLC25A20 with the keyword "Disease variant," indicating potential pathological relevance.
• SLC25A20 contains multiple transmembrane helices and internal repeats, typical of mitochondrial carrier proteins, and is involved in lipid transport across the mitochondrial membrane.

Experimental Guidance and Technical Tips

• For Western blot (WB): Since SLC25A20 is a mitochondrial membrane protein, consider enriching mitochondrial fractions before electrophoresis to enhance signal. The antibody is expected to detect a band at ~33 kDa.
• For immunofluorescence (IF/ICC): Include permeabilization treatments (e.g., Triton X-100) to allow antibody access to the inner mitochondrial membrane. Co-stain with mitochondrial markers (e.g., Tom20) to confirm localization.
• For ELISA: The polyclonal nature may provide high sensitivity; titrate antibody and antigen concentrations for optimal assay conditions.
• Cross-reactivity: The antibody recognizes human and rat SLC25A20; validation in the target species and tissue model is advised before quantitative experiments.
• Note: This is a research-grade polyclonal antibody; for clinical diagnostic applications, further development and validation are required.

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