Anti-SLC25A20 Polyclonal Antibody for WB, ELISA - O43772

Core Product Specifications and Parameters

Biological Background: SLC25A20 Function and Localization

• SLC25A20, also known as carnitine/acylcarnitine translocase (CACT), is a mitochondrial carrier protein belonging to the solute carrier family 25.
• Mediates the electroneutral exchange of acylcarnitines of various chain lengths with free carnitine across the mitochondrial inner membrane via a ping-pong mechanism, a critical step in mitochondrial fatty acid oxidation. Related references: PMID:12892634 PMID:18307102
• Also catalyzes unidirectional transport (uniport) of carnitine, albeit at lower rates than the exchange reaction. Related references: PMID:18307102
• Plays a key role in the carnitine shuttle by delivering acylcarnitines into the mitochondrial matrix where carnitine palmitoyltransferase 2 (CPT-2) releases them for beta-oxidation.
• Localizes to the mitochondrial inner membrane, consistent with its transport function.
• Post-translational modification includes acetylation, and the protein is linked to disease variants (UniProt keyword 'Disease variant'), highlighting its clinical relevance in metabolic disorders.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 1–100 of human SLC25A20, thus the antibody may recognize epitopes in the N-terminal region; consider verifying specificity with peptide blocking.
• In Western blot, a specific band around 33 kDa is expected; validate detection using mitochondrial-enriched lysates from human, mouse, or rat tissues as positive controls.
• For ELISA, both direct and indirect formats can be explored; using the immunizing peptide as a competitor may help confirm specific binding.
• Since cross-reactivity is reported for human, mouse, and rat, pre-test the antibody in your model system to ensure consistent performance across species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research labs with streamlined access to a comprehensive range of IVD raw materials, from validated antibody pairs to rare target sourcing. This anti-SLC25A20 polyclonal antibody targets a crucial mitochondrial transporter, supporting assay development in fatty acid metabolism and related disorders. Reach out to our team for bulk ancillary reagents and tailored sourcing solutions to advance your projects from concept to clinic.