Anti-SLC25A19 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9HC21

Core Product Specifications and Parameters

Biological Background: SLC25A19 Function and Localization

• SLC25A19, also known as mitochondrial thiamine pyrophosphate carrier (MTPPT), is a member of the solute carrier family 25 (SLC25) of mitochondrial transporters.
• It mediates the uptake of thiamine diphosphate (thiamine pyrophosphate) into mitochondria, playing a critical role in cellular energy metabolism.
• The protein functions as an antiporter; however, whether its activity is influenced by mitochondrial membrane potential or proton electrochemical gradient remains unclear.
• Subcellular localization is the mitochondrial membrane, consistent with its transport function.
• Tissue specificity: widely expressed in all tissues examined except placenta, with highest levels in colon, kidney, lung, testis, spleen, and brain.
• Keywords indicate alternative splicing produces multiple isoforms, and post-translational modifications include phosphorylation.
• It contains transmembrane helices, characteristic of the mitochondrial carrier family.

Experimental Guidance and Technical Tips

• For Western blot, the predicted molecular weight is 36kDa; monitor for potential shifts due to post-translational modifications.
• In immunofluorescence/ICC, include a permeabilization step for mitochondrial access and consider co-staining with a mitochondrial marker to confirm localization.
• As a polyclonal antibody recognizing multiple epitopes, verify specificity using siRNA knockdown or appropriate negative controls in your sample system.
• Cross-reactivity with mouse enables application in murine model studies; validate performance in the chosen tissue/cell type.
• For ELISA, optimize coating antigen concentration and detection conditions according to standard protocols.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, connecting diagnostic manufacturers and research labs with high-quality antibodies, antigen pairs, and ancillary reagents. This anti-SLC25A19 polyclonal antibody supports research into mitochondrial transport and metabolic disorders, areas central to many diagnostic applications. Leverage CamelBio's expertise to source validated antibody pairs, bulk monoclonal/polyclonal antibodies, and rare-target raw materials for your entire assay development pipeline.