Anti-SLC25A19 Polyclonal Antibody for WB, IF/ICC, ELISA - Q9HC21

Core Product Specifications and Parameters

Biological Background: SLC25A19 Function and Localization

• SLC25A19 encodes the mitochondrial thiamine pyrophosphate carrier (MTPPT), also known as mitochondrial uncoupling protein 1 (MUP1), and belongs to the solute carrier family 25.
• The protein mediates the uptake of thiamine diphosphate (ThDP) into the mitochondrial matrix, a process that may involve antiporter activity affected by membrane potential or proton electrochemical gradient.
• It is localized to the mitochondrial inner membrane, consistent with its role as a transmembrane transporter.
• The protein contains multiple transmembrane helices and is classified under the mitochondrial carrier (TC 2.A.29) family.
• Expression is detected in a wide range of tissues, with highest levels in colon, kidney, lung, testis, spleen, and brain; it is absent in placenta.
• Post-translational modifications include phosphorylation, and the gene undergoes alternative splicing.
• Mutations in SLC25A19 are associated with neurological disorders, highlighting its importance in neural function.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 1-80 of the human SLC25A19 protein. This N-terminal region is likely exposed on the cytoplasmic side of the outer mitochondrial membrane.
• For Western blot, consider using reducing conditions and a mitochondrial-enriched or whole-cell lysate. The predicted molecular weight is 36 kDa; however, endogenous band patterns may vary due to post-translational modifications.
• In immunofluorescence/ICC, co-staining with mitochondrial markers can help confirm mitochondrial localization.
• ELISA performance should be verified using appropriate recombinant protein or peptide controls.
• Note that cross-reactivity has been reported for human and mouse samples; other species should be validated individually.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to high-quality IVD raw materials. For mitochondrial carrier research and assay development, we offer validated antibody pairs, custom monoclonal/polyclonal antibody generation, and a wide portfolio of ancillary reagents. Whether you need bulk quantities of this anti-SLC25A19 antibody or assistance in sourcing rare target raw materials, CamelBio is your dedicated partner from concept to clinic.