Anti-SLC25A12 Polyclonal Antibody for WB, ELISA - O75746

Core Product Specifications and Parameters

Biological Background: SLC25A12 Function and Localization

• Encoded by the SLC25A12 gene, the protein (also known as Aralar, Aralar1, AGC1) is a member of the mitochondrial carrier family (solute carrier family 25) and functions as an electrogenic aspartate/glutamate antiporter.
• SLC25A12 catalyzes the efflux of aspartate from the mitochondrial matrix in exchange for glutamate and a proton, a key step in the malate-aspartate shuttle that transfers reducing equivalents across the inner mitochondrial membrane. Related references: PMID:11566871 PMID:19641205 PMID:24515575
• The transporter also mediates the uptake of L-cysteinesulfinate (3-sulfino-L-alanine) in exchange for L-glutamate and a proton, and can exchange L-cysteinesulfinate with aspartate without proton translocation. Related references: PMID:11566871
• SLC25A12 lacks detectable transport activity for L-glutamine or gamma-aminobutyric acid (GABA), indicating substrate specificity. Related references: PMID:38945283
• The protein is located in the mitochondrial inner membrane, consistent with its role in metabolite transport across this barrier.
• Tissue-specific expression is highest in heart and skeletal muscle, with lower levels in brain and kidney.
• Mutations in SLC25A12 are associated with developmental and epileptic encephalopathy (DEE39, EIEE39), highlighting its importance in neuronal metabolism.
• UniProt keywords include calcium binding, acetylation, alternative splicing, and disease variant, reflecting post-translational modifications and clinical relevance.

Experimental Guidance and Technical Tips

• For western blotting, the predicted molecular weight is approximately 75 kDa; consider using a suitable membrane (e.g., PVDF) and optimizing blocking and detection conditions for this size range.
• The immunogen corresponds to the N-terminal region (amino acids 1–180); validate antibody specificity in relevant sample systems using appropriate controls such as siRNA knockdown or knockout cell lines.
• In ELISA applications, direct coating of the recombinant or native antigen may be employed; assess cross-reactivity with mouse and rat orthologs if needed.
• Consider the mitochondrial localization of the target when preparing lysates; enriched mitochondrial fractions may improve signal detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. This anti-SLC25A12 polyclonal antibody supports research into mitochondrial transport and metabolic diseases, assay development, and biomarker studies. With technical consulting and supply chain expertise from concept to clinic, CamelBio streamlines your development pipeline.