Anti-SLC25A11 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q02978

Core Product Specifications and Parameters

Biological Background: SLC25A11 Function and Localization

• SLC25A11 (Solute carrier family 25 member 11), also known as 2-oxoglutarate/malate carrier (OGC) or SLC20A4, is a mitochondrial carrier protein belonging to the SLC25 family. It is encoded by the SLC25A11 gene, which gives rise to multiple isoforms through alternative splicing.
• SLC25A11 catalyzes the electroneutral exchange of 2-oxoglutarate (alpha-oxoglutarate) for malate across the inner mitochondrial membrane, a key step in several metabolic shuttles. Related references: PMID:25637873 PMID:38937634
• It can also transport other dicarboxylic acids such as malonate, succinate, maleate, and oxaloacetate with lower affinity, but does not transport glutathione.
• The transport mechanism proceeds via a consecutive exchange model, where one substrate is fully translocated before the second substrate binds for counter-transport. Related references: PMID:38937634
• In addition to its antiport activity, SLC25A11 can facilitate proton transport in the presence of long-chain fatty acid protonophores, contributing to mitochondrial uncoupling and regulation of energetic efficiency.
• SLC25A11 plays essential roles in the malate-aspartate shuttle, oxoglutarate/isocitrate shuttle, gluconeogenesis from lactate, and nitrogen metabolism. It also maintains mitochondrial fusion and fission dynamics and cristae morphology. Related references: PMID:21448454
• The protein is involved in the regulation of apoptosis and is localized exclusively to the inner mitochondrial membrane.
• SLC25A11 is most highly expressed in heart tissue and is subject to post-translational modifications including acetylation and phosphorylation.

Experimental Guidance and Technical Tips

• This rabbit polyclonal antibody was raised against a synthetic peptide derived from the N-terminal region (amino acids 1–100) of human SLC25A11. It is predicted to recognize endogenous SLC25A11 in human, mouse, and rat samples.
• For Western blot (WB), a band at approximately 34 kDa is expected. Due to post-translational modifications or alternative splicing, additional bands may appear; validate the expected molecular weight in your system.
• For IHC-P and IF/ICC, consider including appropriate controls (e.g., peptide blocking or knockdown samples) to confirm specificity. Optimal dilution and incubation conditions should be determined empirically.
• Polyclonal antibodies may exhibit lot-to-lot variability; perform a pilot test when switching to a new lot.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials, including validated antibody pairs, monoclonal/polyclonal antibodies, antigens, and ancillary reagents, to support your diagnostic and research programs from concept to clinic. This anti-SLC25A11 polyclonal antibody is an excellent tool for investigating mitochondrial metabolism, apoptosis, and related disorders. For your project needs, CamelBio also provides tailored sourcing solutions for rare targets and bulk reagent supply.