Anti-SLC25A11 Polyclonal Antibody for WB, ELISA - Q02978

Core Product Specifications and Parameters

Biological Background: SLC25A11 Function and Localization

• SLC25A11, also known as mitochondrial 2-oxoglutarate/malate carrier (OGC), is a member of the solute carrier family 25 that functions as an electroneutral antiporter.
• Catalyzes the exchange of 2-oxoglutarate for malate across the inner mitochondrial membrane, essential for the malate-aspartate shuttle and redox balance (PubMed:25637873, PubMed:38937634). Related references: PMID:25637873, PMID:38937634
• Transports other dicarboxylic acids (malonate, succinate, maleate, oxaloacetate) with lower affinity and facilitates proton leak in the presence of fatty acids, influencing energy efficiency (By similarity).
• Contributes to metabolic pathways including the oxoglutarate/isocitrate shuttle, gluconeogenesis from lactate, and nitrogen metabolism (By similarity).
• Maintains mitochondrial fusion/fission dynamics and cristae structure (PubMed:21448454). Related references: PMID:21448454
• Involved in apoptosis regulation (By similarity).
• Highly expressed in heart, localized to mitochondrial inner membrane.
• Undergoes acetylation, phosphorylation, and alternative splicing.

Experimental Guidance and Technical Tips

• For Western blotting, consider using mitochondrial-enriched fractions or whole cell lysates from human or mouse heart tissue to maximize detection of the 34 kDa protein.
• Validate antibody specificity in the relevant sample system; blocking with the immunogen peptide may help confirm band identity.
• For ELISA, the peptide immunogen suggests potential utility in sandwich or direct ELISA formats; confirm binding to native protein using recombinant SLC25A11 or cell lysates.
• Given the mitochondrial inner membrane localization, ensure sample preparation includes adequate solubilization with non-ionic detergents.
• Cross-reactivity with mouse is indicated, but validation in mouse models or cell lines is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with one-stop access to IVD raw materials, including this anti-SLC25A11 polyclonal antibody for mitochondrial metabolism studies. We supply validated antibody pairs, bulk reagents, and custom sourcing for rare targets, accelerating your assay development from discovery to clinical application.