Anti-SLC25A1 Polyclonal Antibody for WB, IF/ICC, ELISA - P53007

Core Product Specifications and Parameters

Biological Background: Tricarboxylate Transport Protein (SLC25A1) Function and Localization

• SLC25A1 encodes the mitochondrial tricarboxylate transport protein (citrate carrier), a member of the solute carrier family 25, which mediates the electroneutral antiport of citrate and malate across the mitochondrial inner membrane. Related references: PMID:26870663, PMID:29031613, PMID:29238895
• Besides citrate/malate exchange, the carrier can transport isocitrate, phosphoenolpyruvate, cis-aconitate, and, to a lesser extent, trans-aconitate, maleate, and succinate, highlighting its broader substrate flexibility. Related references: PMID:29031613
• Substrate translocation follows a consecutive mechanism where one substrate is fully transported and released before the counter-substrate binds, as revealed by structural and functional studies. Related references: PMID:38937634
• In the cytosol, the imported citrate serves as a precursor for fatty acid and sterol biosynthesis, regulates glycolysis, contributes to protein acetylation, and supports other metabolic and signalling processes. Related references: PMID:29031613, PMID:29238895, PMID:39881208
• The protein is localized to the mitochondrial inner membrane and is a multi-pass transmembrane protein with characteristic carrier repeats.
• Mutations in SLC25A1 are associated with congenital myasthenic syndrome, underlining its essential role in cellular metabolism and neuromuscular function.
• The protein is subject to phosphorylation and has been identified in proteomics studies, reflecting its regulatory and dynamic role in mitochondrial transport.

Experimental Guidance and Technical Tips

• This rabbit polyclonal antibody is raised against full-length human SLC25A1 (amino acids 1–311) and can recognize the endogenous protein across human, mouse, and rat samples. Consider validating reactivity in your specific model system.
• For Western blotting, the predicted molecular weight is ~34 kDa; however, mitochondrial targeting sequences and post-translational modifications may cause slight band shifts. Use appropriate mitochondrial or whole-cell lysates.
• In immunofluorescence/ICC, permeabilization with detergents (e.g., 0.1% Triton X-100) is recommended to access the mitochondrial inner membrane. Co-staining with a mitochondrial marker can confirm localization.
• For ELISA applications, coating the recombinant full-length protein or a specific peptide may improve sensitivity. Include proper positive and negative controls.
• As with any polyclonal antibody, lot-to-lot variation can occur; titrate the antibody for optimal performance in your assay and include batch controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, labs, and research institutes with a comprehensive range of IVD raw materials, technical services, and consulting. For mitochondrial targets like SLC25A1, we can provide validated antibody pairs, optimized monoclonal or polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to accelerate your assay development. Our one-stop model simplifies procurement from concept to clinic, letting you focus on your core research.