Anti-SLC25A1 Polyclonal Antibody for WB, ELISA - P53007

Core Product Specifications and Parameters

Biological Background: SLC25A1 Function and Localization

• SLC25A1 encodes the mitochondrial citrate carrier (CIC), also known as tricarboxylate transport protein, mediating electroneutral antiport of citrate and malate across the mitochondrial inner membrane.
  Related references: PMID:26870663, PMID:29031613, PMID:29238895

• In addition to malate, SLC25A1 can exchange citrate for isocitrate, phosphoenolpyruvate, cis-aconitate, and, to a lesser extent, trans-aconitate, maleate, and succinate.
  Related references: PMID:29031613

• Transport occurs via a sequential mechanism: one substrate is transported first and dissociates from the binding site before the second substrate binds for opposite-direction transport.
  Related references: PMID:38937634

• Cytosolic citrate is a critical precursor for fatty acid and sterol synthesis, regulates glycolysis, and contributes to protein acetylation and other metabolic processes.
  Related references: PMID:29031613, PMID:29238895, PMID:39881208

• The SLC25A1 protein belongs to the mitochondrial carrier (solute carrier family 25) family of transporters and contains characteristic transmembrane domains.

• Localization: SLC25A1 is an integral multi-pass membrane protein of the mitochondrial inner membrane.

• Post-translational modification: Phosphoprotein (by proteomic identification), and genetic variants are linked to congenital myasthenic syndrome and other diseases.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 14-124 of human SLC25A1 (NP_005975.1). This polyclonal antibody likely recognizes linear epitopes, making it suitable for Western blotting under denaturing and reducing conditions.
• For ELISA applications, consider using the immunizing peptide or recombinant SLC25A1 protein as a coating antigen. Include appropriate negative and positive controls to confirm specificity.
• Cross-reactivity is reported for human, mouse, and rat. However, sequence homology should be verified for your specific experimental model; consider performing a sequence alignment of the immunogen region.
• Since SLC25A1 is a mitochondrial membrane protein, ensure adequate sample preparation for WB — use mitochondrial enrichment or whole-cell lysates with suitable detergents to solubilize membrane proteins.
• Given its role as a citrate transporter involved in key metabolic pathways, this antibody may be useful in studying metabolic reprogramming in cancer or metabolic disorders.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw material procurement, providing diagnostic manufacturers and research institutes with one-stop access to validated antibody pairs, monoclonal and polyclonal antibodies, and ancillary reagents. For targets like the mitochondrial citrate transporter SLC25A1, critical in cancer metabolism and congenital disorders, CamelBio bridges the gap from concept to clinic with reliable bulk sourcing and custom raw-material solutions. Let us streamline your pipeline with high-quality reagents and expert support.