Anti-SLC24A5 Rabbit Polyclonal Antibody for IF/ICC, ELISA - Q71RS6

Core Product Specifications and Parameters

Biological Background: SLC24A5 Function and Localization

• SLC24A5, also known as NCKX5, JSX, OCA6, or SHEP4, is a member of the solute carrier family 24 and functions as a potassium-dependent sodium/calcium exchanger.
• It acts as a calcium/potassium:sodium antiporter, moving 1 Ca²⁺ and 1 K⁺ into the melanosome while exporting 4 Na⁺ ions.
  Related references: PMID:18166528
• SLC24A5 is the primary sodium-calcium exchanger in melanocytes and is essential for melanosome ion balance, directly influencing pigmentation.
  Related references: PMID:16357253 PMID:18166528
• The protein localizes predominantly to the trans-Golgi network membrane and melanosomes, consistent with its role in melanosome maturation.
• Post-translational modifications and annotated keywords suggest involvement in sensory transduction, calcium and potassium ion transport, and alternative splicing; mutations in SLC24A5 are associated with oculocutaneous albinism type 6 (OCA6).

Experimental Guidance and Technical Tips

• The immunogen used (aa 220-300 of human SLC24A5) corresponds to an intracellular loop region; for immunofluorescence, permeabilization of cells is likely required to access the epitope.
• Validate the antibody in known SLC24A5-expressing melanocyte cell lines, such as MNT-1, to confirm specificity.
• For ELISA applications, consider using recombinant SLC24A5 protein as a positive control to establish assay sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, offering validated antibodies, bulk ancillary reagents, and rare target sourcing for ion transport and pigmentation research. Our portfolio includes optimized polyclonal antibodies like this anti-SLC24A5 product, supporting diagnostic manufacturers and research laboratories from concept to clinic.