Anti-SLC22A6 Rabbit Polyclonal Antibody for WB and ELISA - Q4U2R8

Core Product Specifications and Parameters

Biological Background: SLC22A6 Function and Localization

• SLC22A6 encodes the organic anion transporter 1 (OAT1), a member of the solute carrier 22 family, with aliases including hOAT1, hPAHT, and hROAT1.
• OAT1 functions as an Na+-independent organic anion/dicarboxylate antiporter, exchanging extracellular organic anions for intracellular dicarboxylates like 2-oxoglutarate. Related references: PMID:11669456, PMID:11907186, PMID:14675047
• Mediates the basolateral uptake of organic anions in kidney proximal tubule epithelial cells, playing a key role in renal clearance. Related references: PMID:9887087
• Transports diverse substrates including prostaglandins (PGE2, PGF2α), cyclic nucleotides (cAMP, cGMP), biopterins, kynurenate, xanthurenate, indoxyl sulfate, and the mycotoxin ochratoxin A. Related references: PMID:11907186, PMID:26377792, PMID:28534121, PMID:22108572, PMID:23832370, PMID:11669456, PMID:14675047
• Subcellular localization is primarily to the basolateral and basal cell membranes.
• Strongly expressed in kidney proximal tubules, with lower expression in liver, skeletal muscle, brain, and placenta. In testis, localized to Sertoli cells. Related references: PMID:10049739, PMID:10462545, PMID:10964714, PMID:9887087, PMID:9950961
• According to UniProt keywords, the protein is glycosylated, undergoes alternative splicing, and has a known 3D structure.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 31–135 of human SLC22A6, a region within the first extracellular/large loop. This design may target an accessible epitope for native transporter recognition.
• For Western blot, consider using kidney tissue lysates or cell lines expressing OAT1. A band of approximately 62 kDa is expected.
• In ELISA applications, the antibody can be used for detection of recombinant or native SLC22A6; coating conditions and detection limits should be optimized empirically.
• Cross-reactivity with rat is noted; however, homology between human and rat SLC22A6 should be examined when designing species-specific experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with ready access to validated raw materials, including antibodies, antigens, and ancillary reagents. This anti-SLC22A6 polyclonal antibody aids drug transporter and renal function studies, and CamelBio can supply additional SLC transporter antibodies along with assay-matched reagent bundles. For specialized requirements, we also source rare-target raw materials through our extensive B2B network.