Anti-SLC22A5 (OCTN2) Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O76082

Core Product Specifications and Parameters

Biological Background: SLC22A5 (OCTN2) Function and Localization

• Encoded by the SLC22A5 gene, this protein is also known as OCTN2, a high-affinity sodium-dependent carnitine transporter that mediates active cellular uptake of carnitine; it also transports organic cations such as tetraethylammonium (TEA) in a sodium-independent manner, with a relative carnitine-to-TEA uptake activity ratio of 11.3. Related references: PMID:10454528, PMID:10525100, PMID:10966938
• In intestinal epithelia, SLC22A5 transports the quorum-sensing pentapeptide CSF from Bacillus subtilis, inducing cytoprotective heat shock proteins and contributing to intestinal homeostasis. Related references: PMID:18005709
• Alternative splicing generates isoforms; one isoform is retained in the endoplasmic reticulum and lacks carnitine transport activity.
• Subcellularly, the transporter localizes to the cell membrane, including apical and basal membranes in polarized cells, and is also detected in the endoplasmic reticulum.
• Highly expressed in kidney, skeletal muscle, heart, placenta, and colon epithelial cells; also present in CD68+ macrophages, CD43+ T-cells, and testicular Sertoli, peritubular myoid, and Leydig cells.
• Post-translational modifications include glycosylation and phosphorylation; mutations in SLC22A5 cause primary systemic carnitine deficiency (OMIM 212140), highlighting its critical role in carnitine homeostasis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal intracellular region (aa 430-529), making this antibody suitable for detecting both unglycosylated and mature forms of the protein.
• Predicted molecular weight is 63kDa; glycosylated or phosphorylated variants may appear as higher molecular weight bands in WB. Appropriate lysis and sample preparation are recommended.
• For IF/ICC, permeabilization is required due to the intracellular epitope; validate staining in known positive tissues (e.g., kidney, heart) with appropriate controls.
• Cross-reacts with mouse and rat SLC22A5; confirm specificity in these species by sequence alignment and experimental validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a reliable one-stop partner for diagnostic manufacturers and research laboratories, offering access to comprehensive IVD raw materials, technical services, and consulting. This anti-SLC22A5 polyclonal antibody is part of our portfolio targeting metabolic and transporter-related targets, supporting studies in carnitine deficiency and intestinal barrier function. From validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents and rare target sourcing, CamelBio streamlines your procurement needs from concept to clinic.