Anti-SLC22A3/OCT3 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - O75751

Core Product Specifications and Parameters

Biological Background: SLC22A3/OCT3 Function and Localization

• SLC22A3 (OCT3/EMT) is a voltage-dependent electrogenic uniporter that transports a broad range of organic cations, including endogenous amines, cationic drugs, and xenobiotics, in a Na⁺- and Cl⁻-independent manner. Related references: PMID:10196521, PMID:10966924, PMID:12538837

• It plays a key role in the clearance and homeostasis of monoamine neurotransmitters such as dopamine, epinephrine, norepinephrine, histamine, serotonin, and tyramine, particularly in the brain. Related references: PMID:10196521, PMID:16581093, PMID:20858707

• SLC22A3 also transports the polyamine agmatine and neuromodulators cyclo(his-pro) and salsolinol, albeit with lower efficiency. Related references: PMID:12538837, PMID:17460754

• The transporter accepts guanidine and choline as substrates and, by sequence similarity, is expected to handle spermidine and putrescine. Related references: PMID:10966924, PMID:40972734

• Subcellularly, SLC22A3 is present at the cell membrane (apical and basolateral domains), as well as on mitochondrial, endomembrane, and nuclear membranes, indicating a potential role in intracellular cation trafficking.

• Tissue distribution is extensive, with expression in liver, intestine, kidney proximal tubules, placenta, brain (multiple regions), testis, respiratory epithelium, skeletal muscle, heart, and adrenal gland, supporting its systemic and organ-specific transport functions.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 301–450, a cytoplasmic loop region predicted to be highly specific for SLC22A3; confirm sequence alignment with orthologs if cross‑reactivity with mouse is desired (this antibody shows cross‑reactivity with mouse).
• For Western blotting (WB), an ~61 kDa band is expected; consider using homogenates from brain, liver, or kidney as positive controls and validate in your specific sample system.
• In immunohistochemistry (IHC‑P), antigen retrieval may be required; test both heat‑induced and enzymatic methods on formalin‑fixed paraffin‑embedded sections of kidney or brain tissue to establish an optimal protocol.
• For ELISA applications, pairing with a capture antibody may be explored; perform standard checkerboard titrations to determine the working concentration.
• As a rabbit monoclonal antibody, it offers high affinity and batch‑to‑batch consistency; validate its performance in your assay using a suitable reference standard.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, labs, and research institutes integrated sourcing of IVD raw materials, including validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. For neuroscience, drug transport, and pharmacokinetic studies, this anti-SLC22A3/OCT3 antibody provides a reliable tool to detect the organic cation transporter OCT3. Our supply network covers rare-target raw materials and supports your workflow from concept to clinic with flexible procurement options.