Anti-SLC22A2 (OCT2) Polyclonal Antibody for WB and ELISA - O15244

Core Product Specifications and Parameters

Biological Background: SLC22A2 Function and Localization

• SLC22A2 encodes organic cation transporter 2 (OCT2), a member of the solute carrier family 22. Alternative splicing produces an isoform that differs in substrate specificity, notably unable to transport guanidine, creatinine, cimetidine, and metformin.
• OCT2 functions as an electrogenic voltage-dependent uniporter mediating Na+-independent, bidirectional transport of various organic cations, driven by membrane potential and concentration gradients (PubMed:9260930, PubMed:9687576, PubMed:21128598, PubMed:15212162). Related references: PMID:9260930 PMID:9687576 PMID:21128598 PMID:15212162
• It plays a critical role in hepatic and renal clearance of cationic drugs and xenobiotics from the bloodstream, primarily localized at the basolateral membrane of hepatocytes and proximal tubules (PubMed:15783073). Related references: PMID:15783073
• In the central nervous system, OCT2 transports monoamine neurotransmitters such as histamine, dopamine, adrenaline, noradrenaline, serotonin, and tyramine, thereby regulating interstitial neurotransmitter concentrations and maintaining dopaminergic cell integrity (PubMed:16581093, PubMed:17460754). Related references: PMID:16581093 PMID:17460754
• Beyond neurotransmitters, OCT2 transports non-amine endogenous compounds including prostaglandins PGE2 and PGF2-alpha, as well as polyamines like agmatine, putrescine, and spermidine (PubMed:11907186, PubMed:12538837, PubMed:21128598). Related references: PMID:11907186 PMID:12538837 PMID:21128598
• The protein is predominantly expressed in kidney, testis, and brain, with additional detection in spleen, placenta, small intestine, spinal cord, and secretory phase endometrium (PubMed:11912245, PubMed:12089365, PubMed:9260930, PubMed:35307651, PubMed:17460754, PubMed:15817714, PubMed:17393420). Related references: PMID:11912245 PMID:12089365 PMID:9260930 PMID:35307651 PMID:17460754 PMID:15817714 PMID:17393420
• Subcellularly, OCT2 localizes to basolateral, basal, and apical cell membranes. Post-translational modifications include glycosylation and phosphorylation, as noted in UniProt keywords.

Experimental Guidance and Technical Tips

• The immunogen encompasses amino acids 44–140 of human SLC22A2, a region potentially distinct among isoforms; users should verify isoform specificity in their experimental context.
• For Western blot, SLC22A2/OCT2 is predicted at ~63 kDa; using reducing conditions and a suitable blocking agent is recommended. Positive control lysates from human kidney or brain tissue may be considered.
• ELISA applications may benefit from using the recombinant immunogen protein as a reference standard for specificity and sensitivity checks.
• Cross-reactivity with rat SLC22A2 has been noted; ortholog sequence alignment should be examined if using this antibody in other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material partner, connecting diagnostic manufacturers and researchers with high-quality antibodies, antigens, and ancillary reagents. For transporter targets like SLC22A2/OCT2, we offer validated polyclonal and monoclonal antibodies, antigen standards, and bulk production support to streamline assay development from concept to clinic. Contact our team for customized sourcing of rare target raw materials and technical consulting.