Anti-SLC22A11 Rabbit Polyclonal Antibody for WB, ELISA - Q9NSA0

Core Product Specifications and Parameters

Biological Background: SLC22A11 Function and Localization

• SLC22A11/OAT4 functions as an organic anion antiporter, mediating the exchange of conjugated steroids (e.g., estrone sulfate, DHEA-S) and urate with dicarboxylates or inorganic ions at the placental basal membrane and renal apical membrane.
  Related references:
  PMID:10660625, PMID:11907186, PMID:15037815, PMID:12409283, PMID:17229912

• It is highly expressed in placental trophoblasts (syncytiotrophoblast and cytotrophoblast) and kidney proximal tubules, consistent with its role in fetal–maternal exchange and renal reabsorption.
  Related references:
  PMID:10660625, PMID:12409283, PMID:15037815, PMID:26277985

• Multiple transport modes have been identified, including E1S/Cl⁻, E1S/OH⁻, urate/Cl⁻, and urate/OH⁻ exchange, as well as DHEA-S/E1S exchange; the protein also mediates unidirectional efflux of glutamate and aspartate.
  Related references:
  PMID:17229912, PMID:28027879

• Beyond endogenous substrates, SLC22A11 transports anionic drugs, diuretics, bile salts, ochratoxin A, and prostaglandins E2 and F2-alpha, suggesting a broader role in xenobiotic clearance and renal excretion.
  Related references:
  PMID:10660625, PMID:11907186, PMID:26277985

• The protein is a glycoprotein with alternative splicing, and it localizes to the cell membrane with apical or basal polarity depending on the tissue.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 40–140 of human SLC22A11, a region that may be partially extracellular; consider verifying epitope accessibility in your assay system and using appropriate membrane protein extraction protocols.
• For Western blot, the predicted molecular weight is ~60 kDa; validate band specificity in relevant human, mouse, or rat tissue/cell lysates.
• The antibody has been reported to cross-react with mouse and rat SLC22A11; if species-specific differences are critical, confirm target recognition in orthologous systems.
• For ELISA applications, optimize coating conditions and antibody dilutions using recombinant protein or transfected cell controls to assess sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, recombinant proteins, and ancillary reagents for every development stage. For transporter targets such as SLC22A11/OAT4, we offer optimized polyclonal antibodies and support assay development with deep technical expertise, streamlining procurement from concept to clinic.