Anti-SLC20A2 Polyclonal Antibody for WB and ELISA - Q08357

Core Product Specifications and Parameters

Biological Background: SLC20A2 Function and Localization

• SLC20A2 functions as a sodium-phosphate symporter, transporting monovalent phosphate with a 2:1 Na⁺:Pi stoichiometry, a key mechanism for cellular phosphate uptake. Related references: PMID:12205090, PMID:15955065, PMID:16790504
• It is critical for bone quality and strength by supplying phosphate for mineralization, and is required to maintain normal cerebrospinal fluid phosphate levels.
• SLC20A2 mediates phosphate-induced calcification of vascular smooth muscle cells and can functionally compensate for loss of the related transporter SLC20A1 in these cells.
• Subcellularly, SLC20A2 localizes to the cell membrane and apical cell membrane, consistent with its role in transmembrane phosphate transport.
• It is ubiquitously expressed across tissues.
• SLC20A2 also serves as a retroviral receptor, conferring susceptibility to infection by amphotropic murine leukemia virus, 10A1 murine leukemia virus, and some feline leukemia virus B variants.
• According to the UniProt record, SLC20A2 is a phosphoprotein and glycoprotein, with disease-associated variants linked to idiopathic basal ganglia calcification (IBGC), as reflected in aliases such as IBGC1, IBGC2, and IBGC3.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 235–485 of human SLC20A2 (isoform NP_001244110.1), a region that includes predicted intracellular loops; consider potential accessibility differences between native and denatured protein in your assay.
• For western blotting, a band around 70 kDa is expected; verify specificity using appropriate controls and consider testing a range of antibody dilutions for optimal signal.
• This antibody is reported to cross-react with mouse and rat SLC20A2; however, validation in the relevant species and sample type is recommended.
• ELISA applications can be developed using recombinant protein standards; consider optimizing coating and detection conditions for your specific experimental setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with reliable access to IVD raw materials, including this SLC20A2 polyclonal antibody for phosphate transport and viral entry studies. Our supply program covers validated antibody pairs, bulk reagents, and sourcing for rare targets to support your assay development from concept to clinic.