Anti-SLC1A7 Rabbit Polyclonal Antibody for WB, ELISA - O00341

Core Product Specifications and Parameters

Biological Background: SLC1A7 Function and Localization

• SLC1A7 encodes the excitatory amino acid transporter 5 (EAAT5), also known as the retinal glutamate transporter, a member of the solute carrier family 1.
• EAAT5 is a sodium-dependent, high-affinity amino acid transporter mediating uptake of L-glutamate, L-aspartate, and D-aspartate. It functions as a symporter, co-transporting one amino acid with two or three Na+ ions and one proton, in exchange for one K+ ion. Related references: PMID:9108121
• In addition to transport, EAAT5 acts as an inhibitory glutamate-gated chloride channel, serving as a major inhibitory presynaptic receptor at mammalian rod bipolar cell axon terminals, thereby influencing visual processing.
• The protein localizes to the photoreceptor inner segment membrane and synaptic cell membrane.
• SLC1A7 is predominantly expressed in the retina, with lower detectable levels in liver, heart, muscle, and brain.
• EAAT5 is a glycoprotein and multi-pass membrane protein, and alternative splicing generates multiple transcript variants.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 480–560) of human SLC1A7; consider verifying whether the antibody detects both the canonical 60 kDa protein and potential shorter isoforms (e.g., ~17 kDa) in relevant samples.
• For Western blotting, validate the antibody in retinal tissue or cell lines expressing SLC1A7, such as mouse retina, taking advantage of the reported mouse cross-reactivity.
• For ELISA applications, the recombinant immunogen can serve as a positive control to assess antibody binding and sensitivity.
• Since SLC1A7 is expressed at lower levels in liver, heart, muscle, and brain, use appropriate loading controls and enrichment strategies when probing these tissues.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes a streamlined, one-stop channel for IVD raw-material procurement. The Anti-SLC1A7 Rabbit Polyclonal Antibody (O00341) supports investigations into glutamate transport and retinal synaptic physiology, complementing CamelBio’s portfolio of validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Through its extensive sourcing network, CamelBio also facilitates access to rare-target raw materials, helping to accelerate your project from concept to clinic.