Anti-SLC1A7 Rabbit Polyclonal Antibody for WB and ELISA - O00341

Core Product Specifications and Parameters

Biological Background: SLC1A7 Function and Localization

• Excitatory amino acid transporter 5 (EAAT5), also known as solute carrier family 1 member 7 (SLC1A7) or retinal glutamate transporter, is encoded by the SLC1A7 gene in humans.
• Functions as a sodium-dependent, high-affinity amino acid transporter mediating the uptake of L-glutamate, L-aspartate, and D-aspartate; acts as a symporter with 2-3 Na+ ions and one proton, counter-transporting K+. Related references: PMID:9108121
• Additionally serves as an inhibitory glutamate-gated chloride channel, mediating a large Cl- conductance that affects visual processing at mammalian rod bipolar cell axon terminals (by similarity).
• Localizes to the photoreceptor inner segment membrane and synaptic cell membrane, consistent with its role in retinal signaling.
• Predominantly expressed in the retina, with detectable levels also in liver, heart, muscle, and brain, implying potential extra-retinal functions.
• Subject to alternative splicing and glycosylation, which may regulate its transport activity or subcellular distribution.

Experimental Guidance and Technical Tips

• The immunogen targets amino acids 480-560 within the C-terminal region of human SLC1A7; consider verifying isoform specificity in your experimental system.
• For Western blot, a band around 61 kDa is expected; validate with appropriate positive and negative controls to confirm specificity.
• Reported mouse cross-reactivity suggests utility in rodent models; always validate in your specific tissue or cell line.
• ELISA applications may require optimization of coating antigen and antibody concentration; include relevant controls for accurate quantification.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive IVD raw material solutions, from validated antibody pairs and optimized polyclonal antibodies to bulk ancillary reagents, serving diagnostic manufacturers and researchers worldwide. Our portfolio supports glutamate transporter and retinal studies, and we facilitate custom sourcing for rare targets like SLC1A7, ensuring seamless integration from concept to clinic.