Anti-SLC1A6 Rabbit Polyclonal Antibody for WB and ELISA - P48664

Core Product Specifications and Parameters

Biological Background: SLC1A6 Function and Localization

• SLC1A6 encodes excitatory amino acid transporter 4 (EAAT4), a sodium-dependent high-affinity transporter that mediates uptake of L-glutamate, L-aspartate, and D-aspartate. Related references: PMID:26690923, PMID:7791878
• EAAT4 functions as a symporter, co-transporting one amino acid molecule with two or three Na+ ions and one proton, while counter-transporting one K+ ion. Related references: PMID:7791878
• EAAT4 mediates an uncoupled chloride flux that prevents charge accumulation during amino acid transport. Related references: PMID:7791878
• It is predominantly expressed in the brain, with dense and selective localization in the cerebellum, specifically in Purkinje cell bodies. Related references: PMID:7791878
• The protein localizes to the cell membrane, consistent with its role in synaptic glutamate clearance.
• Post-translational modifications include phosphorylation (Phosphoprotein keyword) and glycosylation (Glycoprotein keyword).
• Alternative splicing generates multiple isoforms (Alternative splicing keyword).
• It belongs to the solute carrier family 1 (SLC1) and is also known as sodium-dependent glutamate/aspartate transporter.

Experimental Guidance and Technical Tips

• The immunogen spans residues 155–265, likely an intracellular loop; consider this for blocking peptide design or epitope mapping.
• For Western blot, validate in human and mouse brain lysates, particularly cerebellum where EAAT4 is abundant in Purkinje cells.
• In ELISA, use recombinant EAAT4 protein or peptide antigens to confirm specificity and optimize antibody titration.
• Check sequence alignments for conserved regions among EAAT family members to assess potential cross-reactivity.
• Include appropriate controls (e.g., knockout cell lines or siRNA knockdown) to confirm target specificity.

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CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. Our portfolio includes validated antibody pairs, optimized polyclonal antibodies like this anti-SLC1A6 pAb, and bulk ancillary reagents for neuroscience and transporter research. For rare targets or custom raw-material sourcing, our team ensures reliable supply chain solutions to accelerate your diagnostic development.