Anti-SLC1A5 Rabbit Polyclonal Antibody for WB, ELISA - Q15758

Core Product Specifications and Parameters

Biological Background: SLC1A5 Function and Localization

• SLC1A5, also known as Neutral amino acid transporter B(0) or ASCT2, is a sodium-coupled neutral amino acid antiporter that mediates the bidirectional exchange of amino acids, with L-glutamine as the preferred substrate, coupling its transport to at least one sodium ion. Related references: PMID:17094966; PMID:23756778; PMID:26492990
• Through L-glutamine uptake, SLC1A5 fuels biosynthesis of proteins, nucleotides, and amine sugars and supports mitochondrial oxidative phosphorylation, driving the metabolic switch toward cell differentiation in stem and activated cells. Related references: PMID:23756778; PMID:24953180
• It transports extracellular L-glutamate in a pH-dependent manner, co-transporting one proton and one sodium ion in exchange for intracellular L-glutamine, thereby participating in the glutamine/glutamate cycle in the nervous system. Related references: PMID:32733894
• SLC1A5 mediates the release and reabsorption of D-serine from retinal glia and renal proximal tubules, respectively, modulating NMDA receptor function and D-serine homeostasis. Related references: PMID:17094966; PMID:38650461
• The transporter also displays an uncoupled, sodium- and amino acid-dependent channel-like anion conductance with a preference for SCN(-), NO3(-), I(-), and Cl(-).
• Beyond its transport function, SLC1A5 acts as a cell surface receptor for several retroviruses, including feline endogenous virus RD114, baboon M7 virus, and type D simian retroviruses, and mediates syncytin-1-induced trophoblast fusion in placental development. Related references: PMID:10708449; PMID:23492904; PMID:38671230
• Subcellularly, it is found at the cell membrane and apical membrane, and is also identified in melanosomes, with tissue expression in placenta, lung, skeletal muscle, kidney, pancreas, intestine, and CD34+ hematopoietic progenitors. Related references: PMID:8702519; PMID:24953180

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 154–224 within the central region of human SLC1A5, which may favor recognition of the native topology if epitopes are accessible; consider evaluating binding in both denaturing and native conditions if needed.
• For Western blotting, the predicted molecular weight is approximately 57 kDa; due to glycosylation and possible dimerization, observed bands may shift; validate using positive control lysates from placenta or lung tissues.
• The reported cross-reactivity with human, mouse, and rat suggests conservation of the epitope region; confirm reactivity in your specific experimental model by running a species-matched positive control.
• ELISA applications may require optimization of coating antigen and antibody concentration; consider using the recombinant immunogen as a standard.
• As a rabbit polyclonal antibody, batch-to-batch variability is possible; include internal reference controls and re-validate when switching to a new lot.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is dedicated to supporting your SLC1A5 research and assay development with seamless access to this polyclonal antibody and complementary IVD raw materials. Whether you need matched antibody pairs for ELISA implementation, bulk quantities of ancillary reagents, or custom sourcing for rare targets, our integrated supply chain accelerates your transition from research to diagnostic application.