Anti-SLC1A5 Rabbit Polyclonal Antibody for WB and ELISA - Q15758

Core Product Specifications and Parameters

Biological Background: SLC1A5 Function and Localization

• SLC1A5, also known as ASCT2, is a sodium-coupled neutral amino acid antiporter with a strong preference for L-glutamine, exchanging extracellular neutral amino acids for intracellular counterparts in a tri-substrate transport cycle (PubMed:17094966, PubMed:23756778, PubMed:26492990). Related references: PMID:17094966, PMID:23756778, PMID:26492990
• It plays a critical role in supplying glutamine to proliferating cells, including cancer cells and activated T cells, driving metabolic reprogramming and cell differentiation (PubMed:23756778, PubMed:24953180). Related references: PMID:23756778, PMID:24953180
• SLC1A5 functions as a cell surface receptor for several retroviruses, including feline endogenous virus RD114, baboon M7 endogenous virus, and type D simian retroviruses, facilitating viral entry and syncytialization (PubMed:10708449, PubMed:23492904, PubMed:38671230). Related references: PMID:10708449, PMID:23492904, PMID:38671230
• The protein localizes to the plasma membrane, with enrichment at the apical membrane in polarized cells and in melanosomes.
• SLC1A5 is widely expressed in placenta, lung, skeletal muscle, kidney, pancreas, and intestine, and is notably present in CD34+ hematopoietic progenitors, where it facilitates glutamine uptake essential for differentiation and proliferation (PubMed:24953180). Related references: PMID:24953180
• It is a glycoprotein subject to acetylation and phosphorylation, and undergoes alternative splicing and initiation.
• SLC1A5 mediates D-serine release from retinal glia and contributes to renal D-serine reabsorption, potentially modulating NMDA receptor function and urinary D-serine dynamics (PubMed:17094966, PubMed:38650461). Additionally, it exhibits a sodium- and amino acid-dependent uncoupled anion conductance with a preference for SCN(-) >> NO3(-) > I(-) > Cl(-). Related references: PMID:17094966, PMID:38650461

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-100) of human SLC1A5, which may be conserved across isoforms; consider this when designing detection strategies.
• For Western blot, validate the antibody using lysates from tissues or cell lines with known SLC1A5 expression (e.g., placenta, lung, or cancer cells). A band of approximately 57 kDa is expected.
• For ELISA, optimize coating and detection conditions using recombinant SLC1A5 protein or peptide standards.
• Cross-reactivity with rat SLC1A5 has been observed; this antibody may be suitable for rat model studies after appropriate validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted partner for one-stop IVD raw material sourcing, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. Our anti-SLC1A5 antibody supports research into amino acid transport, cancer metabolism, and viral receptor biology, with comprehensive supply solutions from concept to clinic. Whether you need rare target raw materials or technical consulting, CamelBio accelerates your development journey.