Anti-SLC1A4 Polyclonal Antibody for WB, IHC-P, ELISA - P43007

Core Product Specifications and Parameters

Biological Background: SLC1A4 Function and Localization

• SLC1A4 (ASCT-1) is a sodium-coupled neutral amino acid antiporter that exchanges substrates such as L- and D-serine, L-threonine, L-asparagine, and L-alanine across the plasma membrane (PubMed:14502423, PubMed:26041762, PubMed:27272177). Related references: PMID:14502423 PMID:26041762 PMID:27272177
• It plays a critical role in D-serine homeostasis at excitatory synapses, mediating electrogenic D-serine influx in astrocytes coupled to L-serine efflux, which supports neuronal de novo D-serine synthesis (By similarity).
• In addition to transport, SLC1A4 exhibits uncoupled channel-like anion conductance with a selectivity sequence of SCN(-) > NO3(-) > I(-) > Cl(-) (PubMed:8910405). Related references: PMID:8910405
• The protein localizes to the cell membrane and melanosome membrane.
• It is highly expressed in brain, muscle, and pancreas, with ubiquitous low-level expression across all tissues.
• SLC1A4 is subject to post-translational modifications including glycosylation, phosphorylation, and acetylation, and genetic variants are linked to neurological disorders such as epilepsy and intellectual disability.

Experimental Guidance and Technical Tips

• For Western blot (WB), expect a band at approximately 56 kDa. Consider using positive controls from brain or pancreas lysates where SLC1A4 is highly expressed.
• In immunohistochemistry (IHC-P), antigen retrieval steps may be necessary; validate the antibody in the relevant tissue type with appropriate controls.
• For ELISA, peptide competition assays can help confirm binding specificity. Since this is a polyclonal antibody, monitor lot-to-lot consistency and titrate as needed.
• The antibody shows cross-reactivity with mouse and rat, making it suitable for translational studies in rodent models; however, always verify performance in your specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with a comprehensive portfolio of IVD raw materials, including validated antibodies, bulk ancillary reagents, and rare target sourcing for targets like SLC1A4. Our one-stop platform streamlines procurement from concept to clinic, offering technical consulting and customized supply solutions to accelerate your amino acid transport and neurological research assays. Whether you need antibody pairs or large-scale production, we are your trusted partner.