Anti-SLC19A1 (Reduced Folate Transporter) Rabbit Polyclonal Antibody for WB, ELISA - P41440

Core Product Specifications and Parameters

Biological Background: SLC19A1 (Reduced Folate Transporter) Function and Localization

• SLC19A1, also known as reduced folate transporter (RFC), hRFC, intestinal folate carrier 1 (IFC-1), or RFT-1, is encoded by the SLC19A1 gene (also FLOT1, RFC1) and belongs to the solute carrier family 19.
• Functions as an antiporter that mediates the import of reduced folates and a subset of cyclic dinucleotides, driven by intracellular organic anion export. Related references: PMID:10787414, PMID:15337749, PMID:16115875
• Imports immunoreactive cyclic dinucleotides, including 2'-3'-cGAMP and 3'-3'-cGAMP, thereby contributing to immune responses following cytosolic DNA virus detection. Related references: PMID:31126740, PMID:31511694, PMID:36745868
• Has high affinity for N5-methyltetrahydrofolate, the predominant circulating folate, and also transports the antifolate drug methotrexate. Related references: PMID:10787414, PMID:14609557, PMID:22554803, PMID:36071163, PMID:7615551
• Localizes primarily to the cell membrane, with expression at both apical and basolateral surfaces.
• Exhibits highest expression in placenta and liver, with lower levels in lung.
• Post-translationally modified by acetylation and phosphorylation; classified as a glycoprotein and linked to hereditary hemolytic anemia per UniProtKB keywords.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 452–591) of human SLC19A1, which may help in detecting full-length protein in Western blotting at approximately 65 kDa.
• For Western blot, validate the antibody in the relevant sample system; consider using placenta or liver lysates, which express high levels of SLC19A1.
• In ELISA applications, optimize coating antigen and detection conditions; the antibody can be used for direct antigen detection when paired with appropriate secondary antibodies.
• Cross-reactivity is expected with human SLC19A1; for other species, verify sequence homology before use.

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CamelBio serves as your comprehensive procurement partner for IVD raw materials, offering validated antibodies like this anti-SLC19A1 polyclonal antibody for folate transporter and immuno-oncology research. We provide one-stop access to antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets, supporting your diagnostic development from concept to clinic.