Anti-SLC18A3 Rabbit Polyclonal Antibody for IF-P, IHC-P, ELISA - Q16572

Core Product Specifications and Parameters

Biological Background: SLC18A3 Function and Localization

• SLC18A3, also known as vesicular acetylcholine transporter (VACHT), belongs to the solute carrier 18 family and is primarily responsible for loading acetylcholine into synaptic vesicles.
• It functions as an electrogenic antiporter that exchanges one cholinergic neurotransmitter (acetylcholine or choline) with two intravesicular protons, utilizing the proton gradient established by vacuolar-type H+-ATPase. Related references: PMID:20225888, PMID:8910293
• The transporter determines cholinergic vesicular quantal size, which influences motor neuron differentiation and innervation patterning at developing neuromuscular junctions.
• In the forebrain cholinergic system, SLC18A3 regulates hippocampal synaptic transmission and is essential for spatial memory formation.
• Besides acetylcholine and choline, SLC18A3 can also transport serotonin, indicating broader substrate specificity.
• Subcellularly, it localizes to the membrane of cytoplasmic synaptic vesicles, consistent with its role in neurotransmitter storage.
• Tissue expression is restricted to peripheral and central cholinergic neurons, making it a specific marker for cholinergic systems.
• Mutations in SLC18A3 are associated with congenital myasthenic syndrome, highlighting its clinical importance in neurotransmission.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 473–532) of human SLC18A3; perform sequence alignment to verify epitope conservation when using other species such as mouse or rat.
• For immunofluorescence (IF-P) and immunohistochemistry (IHC-P), optimal detection may require optimized fixation and antigen retrieval; validate using positive control tissues (e.g., brain sections from cholinergic regions).
• The antibody reacts with mouse and rat orthologs, making it suitable for cross-species studies, but confirm cross-reactivity in your specific experimental system.
• In ELISA applications, consider pairing this antibody with appropriate secondary detection reagents and validate assay conditions for your target analyte.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutions a comprehensive one-stop platform for IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For cholinergic neurotransmission and synaptic vesicle research, we provide targeted tools like this anti-SLC18A3 antibody. Our sourcing network also assists with rare target raw-material procurement and technical consulting to accelerate your projects from concept to clinic.