Anti-SLC17A7 Polyclonal Antibody for WB, IF/ICC, IF-P, ELISA - Q9P2U7

Core Product Specifications and Parameters

Biological Background: SLC17A7 Function and Localization

• SLC17A7 encodes vesicular glutamate transporter 1 (VGLUT1), a member of the solute carrier 17 family predominantly expressed in brain regions including the amygdala, cerebellum, cerebral cortex, and hippocampus.
• VGLUT1 functions as a multifunctional transporter that mediates the vesicular uptake of L-glutamate and also transports chloride, protons, potassium, sodium, and phosphate ions (PubMed:10820226). Related references: PMID:10820226
• At the synaptic vesicle membrane, it primarily operates as an electrogenic L-glutamate uniporter, powered by the proton electrochemical gradient established by vacuolar H(+)-ATPase.
• The protein exhibits ion channel and antiporter activities, including a chloride channel and a K(+)/H(+) antiporter, which modulate synaptic vesicle acidification and sustain glutamate loading.
• Following exocytosis, VGLUT1 localizes to the plasma membrane and acts as an Na(+)/phosphate symporter, contributing to synaptic phosphate homeostasis (PubMed:10820226). Related references: PMID:10820226
• Subcellularly, VGLUT1 is found in the synaptic vesicle membrane, plasma membrane, and synaptosomes, consistent with its roles in neurotransmitter storage and phosphate regulation.
• The protein is essential for normal visual signaling, being required for synaptic transmission of visual-evoked responses from photoreceptors.
• Keywords include alternative splicing, phosphoprotein, and various transport activities, indicating regulatory complexity and multiple functional states.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 461–560) of human SLC17A7; the antibody may detect full-length protein and C-terminal fragments or splice variants.
• For Western blotting, expect a band near 62 kDa; additional bands may arise from post-translational modifications or alternative splicing. Validate specificity using appropriate controls.
• In immunofluorescence and immunocytochemistry, optimize fixation and permeabilization for the target tissue or cell line, as VGLUT1 is embedded in synaptic vesicle membranes.
• When performing ELISA, consider pairing this polyclonal antibody with a suitable monoclonal antibody for sandwich assays or use it for direct antigen capture.
• Verify cross-reactivity in mouse samples, as the antibody is reported to recognize both human and mouse SLC17A7.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines the procurement of high-quality IVD raw materials, connecting diagnostic manufacturers and researchers with rigorously validated reagents like rabbit polyclonal antibodies against SLC17A7/VGLUT1. Our comprehensive portfolio includes antibody pairs, recombinant proteins, and ancillary reagents, all backed by technical and regulatory consult to accelerate neuroscience and neurobiology assay development.