Anti-SLC17A6 Rabbit Polyclonal Antibody for WB, IF-P, ELISA - Q9P2U8

Core Product Specifications and Parameters

Biological Background: SLC17A6 Function and Localization

• SLC17A6 (VGLUT2) is a vesicular glutamate transporter that loads L-glutamate into synaptic vesicles via an electrogenic uniport mechanism driven by the proton electrochemical gradient. It also transports phosphate and functions as a chloride channel and K+/H+ antiporter to regulate vesicular acidification and membrane potential. Related references: PMID:11698620; PMID:33440152
• At the plasma membrane, SLC17A6 mediates Na+-coupled phosphate uptake, contributing to synaptic phosphate homeostasis. Related references: PMID:10820226
• Predominantly expressed in adult brain, with high levels in regions such as the amygdala, hippocampus, cerebral cortex, and thalamus. Related references: PMID:10820226
• Subcellularly, SLC17A6 localizes to the synaptic vesicle membrane, synaptosomes, and can transiently appear on the cell surface following exocytosis.
• It belongs to the solute carrier family 17 and is predicted to have multiple transmembrane helices, typical for a transport protein.
• Post‑translational modifications include glycosylation, which may affect antibody detection.
• Beyond the central nervous system, SLC17A6 may play roles in the endocrine glutamatergic system of the pineal gland and pancreas, and in retinal vascular development.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide covering amino acids 500‑582 (NP_065079.1). This C‑terminal region is conserved across human, mouse, and rat, consistent with observed cross‑reactivity.
• Predicted molecular weight is ~64 kDa; however, glycosylation may lead to a higher apparent band on Western blots. Consider using positive control lysates from brain tissues.
• For immunofluorescence, permeabilization is required to access the intracellular epitope; optimize fixation and detergent conditions for synaptic vesicle localization.
• Suitable for ELISA applications; antibody concentration should be titrated for each assay format.
• Validate specificity in knockout or knockdown models to confirm target‑specific signal.

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