Anti-SLC16A7 (MCT2) Rabbit Polyclonal Antibody for WB, ELISA - O60669

Core Product Specifications and Parameters

Biological Background: SLC16A7 (MCT2) Function and Localization

• SLC16A7 (MCT2) is a proton-coupled monocarboxylate symporter that mediates the rapid plasma membrane transport of key energy substrates, including L-lactate, pyruvate, and ketone bodies (acetoacetate, beta-hydroxybutyrate, and acetate). Functional transport requires dimerization, with both subunits working cooperatively. Related references: PMID:32415067 PMID:9786900
• The protein is localized at the cell membrane, specifically the basolateral membrane in polarized cells, and is also found in the cytoplasm.
• Tissue expression is highest in testis; moderate to low levels are detected in spleen, heart, kidney, pancreas, skeletal muscle, brain, and leukocytes. While expression is restricted in normal tissues, MCT2 is widely up-regulated in cancer cells. Related references: PMID:15505343 PMID:9786900
• Structural features include 12 transmembrane helices, and the protein has been crystallized, providing a reference model for the monocarboxylate transporter family (UniProt keyword: 3D-structure).
• MCT2 facilitates the uptake and distribution of monocarboxylates, supporting cellular energy metabolism, especially in tissues with high energy demands.

Experimental Guidance and Technical Tips

• For Western blotting (WB), expect a band at approximately 52 kDa. Consider using human testis or heart lysate, or rat tissue extracts, as positive controls.
• In ELISA applications, the immunogen-derived peptide may be utilized as a reference standard; validate the antibody as a capture or detection reagent in your specific sample matrix.
• As a polyclonal antibody, lot-to-lot consistency should be monitored. Determine optimal working dilutions empirically for each experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive sourcing for IVD raw materials, from research-grade antibodies to bulk production lots. For monocarboxylate transporter targets such as SLC16A7/MCT2, we offer validated antibody pairs, optimized polyclonal and monoclonal antibodies, and ancillary reagents to support your diagnostic assay development. Leverage our integrated technical and consulting services to accelerate your project from concept to clinic.