Anti-SLC16A4 Rabbit Polyclonal Antibody for WB, ELISA - O15374

Core Product Specifications and Parameters

Biological Background: SLC16A4 Function and Localization

• SLC16A4 encodes the probable monocarboxylate transporter 5, also named MCT4 or MCT5.
• It belongs to the solute carrier family 16, which facilitates the proton-linked transport of monocarboxylates such as lactate and pyruvate across plasma membranes.
• The protein is predicted to localize primarily at the cell membrane, consistent with its transporter role.
• Uniprot keywords indicate it contains multiple transmembrane helices, supporting its integration into the lipid bilayer.
• Alternative splicing of the SLC16A4 gene may generate multiple isoforms, as suggested by the detected transcript variants.
• Functional annotation suggests it may operate as a symporter, coupling monocarboxylate movement with other ions like protons.
• Mass spectrometry-based proteomics has identified SLC16A4 expression in various human tissues.
• The theoretical molecular weight of the canonical isoform is approximately 54 kDa.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide mapping to the C-terminal region (aa 388–487). This design favors detection of the full-length protein and may reduce cross-reaction with other MCT family members.
• For Western blot, consider running a nuclear-free membrane extract and using a mild detergent lysis buffer to preserve membrane protein integrity.
• The antibody is qualified for ELISA. When developing a sandwich ELISA, pairing with a capture antibody targeting a distinct epitope may improve sensitivity.
• Cross-reactivity with mouse is indicated; however, validation in the relevant sample system (e.g., mouse tissue lysates) is recommended.
• As with any polyclonal antibody, lot-to-lot consistency should be verified if batch switching is planned.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD manufacturers and research laboratories, offering a streamlined supply of raw materials from concept to clinic. This SLC16A4 antibody supports investigations into monocarboxylate transport and metabolic regulation, and CamelBio can provide matched antibody pairs, bulk quantities, and ancillary reagents for assay development. For projects requiring rare or custom targets, our team assists in efficient raw-material identification and procurement to accelerate your diagnostic workflow.