Anti-SLC16A4 Rabbit Polyclonal Antibody for WB and ELISA - O15374

Core Product Specifications and Parameters

Biological Background: SLC16A4 Function and Localization

• SLC16A4 encodes the probable monocarboxylate transporter 5, also designated MCT4 (Monocarboxylate Transporter 4) or MCT5, a member of the solute carrier family 16.
• The protein functions as a proton‑coupled monocarboxylate symporter, mediating the uptake and efflux of metabolically important monocarboxylates such as lactate, pyruvate, and ketone bodies across the plasma membrane.
• SLC16A4 is localized to the cell membrane, consistent with its role in transmembrane transport.
• Structural predictions indicate the protein contains multiple transmembrane helices, forming a transport pore that couples substrate movement to the proton gradient.
• The gene undergoes alternative splicing, giving rise to multiple transcript variants that may contribute to functional diversity.
• As a transporter of monocarboxylates, SLC16A4 participates in key physiological processes including pH regulation, energy metabolism, and intercellular metabolite shuttling.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 388–487 of human SLC16A4; this C‑terminal region may confer selectivity for the target protein, but validation in your specific sample system is recommended.
• For Western blotting, the predicted molecular weight is approximately 54 kDa; consider using whole‑cell lysates from human or rat cell lines known to express SLC16A4, with appropriate loading controls.
• As a membrane protein, sample preparation should include suitable solubilization and denaturing conditions to maintain integrity and accessibility of the epitope.
• In ELISA, the peptide‑derived immunogen may yield high specificity; titration of the antibody and optimization of coating and blocking conditions are advised.
• Being a polyclonal antibody, it may detect multiple isoforms or related family members; confirm specificity by knockdown or comparison with known negative tissues.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves diagnostic manufacturers, laboratories, and research institutes with a complete portfolio of IVD raw materials and technical support—from concept to clinic. For your SLC16A4 / monocarboxylate transporter projects, we supply validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare‑target raw‑material sourcing. Partner with us to streamline procurement and accelerate your development timelines.