Anti-SLC16A3/MCT4 Rabbit Monoclonal Antibody for WB, IF/ICC, IHC-P, ELISA - O15427

Core Product Specifications and Parameters

Biological Background: SLC16A3/MCT4 Function and Localization

• SLC16A3 (MCT4) is a proton-coupled monocarboxylate transporter that mediates the bidirectional transport of L-lactate and pyruvate across the plasma membrane. Related references: PMID:11101640, PMID:23935841, PMID:31719150
• Plays a crucial role in L-lactate efflux from highly glycolytic cells, such as cancer cells and fast-twitch muscle fibers, thereby regulating intracellular pH and metabolic cooperation.
• The protein is highly expressed in skeletal muscle, where it facilitates lactate export during anaerobic glycolysis, preventing intracellular acidification.
• Localized to the cell membrane, specifically the basolateral membrane in polarized cells, enabling vectorial transport of metabolites.
• MCT4 belongs to the solute carrier family 16 (SLC16), which comprises 14 members, with MCT4 being the fourth isoform (also designated MCT3 in earlier nomenclature).
• Post-translational modifications include phosphorylation, as indicated by proteomics identification and phosphoprotein keywords, which may regulate its activity or trafficking.
• Keywords: cell membrane, membrane, symport, transmembrane, transmembrane helix, transport – consistent with its role as an integral membrane transporter.
• The protein has a molecular weight of approximately 49 kDa (calculated from sequence) and contains 12 predicted transmembrane domains.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 410-465 of human SLC16A3/MCT4 (NP_004198.1), a region near the C-terminus. This design may confer specificity across MCT4 splice variants or isoforms; however, validation in the relevant species and sample system is recommended.
• For western blot (WB), the predicted molecular weight is ~49 kDa, but post-translational modifications or oligomerization may result in additional bands; consider optimizing reducing conditions and blocking buffers.
• In immunofluorescence (IF/ICC) and immunohistochemistry (IHC-P), membrane localization is expected; ensure adequate permeabilization and test fixation methods to preserve epitope accessibility.
• For ELISA, consider using the recombinant protein immunogen as a positive control to establish standard curves and assay sensitivity.
• This antibody is reported to react with human SLC16A3; cross-reactivity with other species or closely related monocarboxylate transporters (e.g., MCT1) has not been determined. Users should validate species and target specificity independently.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized recombinant antibodies, and bulk ancillary reagents to accelerate your diagnostic development. As the supplier of this anti-SLC16A3/MCT4 rabbit monoclonal antibody, we support cancer metabolism, lactate signaling, and pH regulation research with reliable tools and raw-material solutions. Our global sourcing network also enables rapid access to rare targets, ensuring your projects move seamlessly from concept to clinic.