Anti-SLC14A1/Urea Transporter 1 Polyclonal Antibody for WB, IHC-P, IF-P, ELISA - Q13336

Core Product Specifications and Parameters

Biological Background: SLC14A1 (Urea Transporter 1) Function and Localization

• SLC14A1 encodes urea transporter 1 (UT-B1), a transmembrane protein that mediates the transport of urea across cell membranes driven by a concentration gradient. This activity is essential for erythrocyte urea permeability and urinary concentration in the kidney.
  Related references:
  PMID:10514515 PMID:7797558 PMID:7989337
• The protein also facilitates water transport in erythrocytes, contributing to cell volume regulation (By similarity).
• It belongs to the solute carrier family 14 (urea transporter) and is a member of the urea transporter (UT) family.
• SLC14A1 is located in the cell membrane, with basolateral localization in renal collecting duct cells, positioning it for transepithelial urea movement.
• The protein carries the JK blood group antigens (Jk(a) and Jk(b)), making it clinically significant in transfusion medicine.
• SLC14A1 is predominantly expressed in erythrocytes and the kidney, with detection in spleen erythroblasts and certain kidney tumors.
  Related references:
  PMID:23219802 PMID:7989337
• Predicted to undergo N-linked glycosylation, which may influence its stability and trafficking.
• Alternative splicing generates multiple isoforms, though their functional differences are not fully characterized.

Experimental Guidance and Technical Tips

• The immunogen corresponds to an internal region (amino acids 158-257) of human SLC14A1, which may recognize both glycosylated and unglycosylated forms of the protein.
• For Western blotting (WB), a band around 43 kDa is expected; consider using protease inhibitors during sample preparation to avoid degradation.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF-P), membrane localization may require optimized permeabilization and antigen retrieval conditions.
• ELISA can be employed for antibody titering or as a capture/detection reagent in sandwich assays; validate sensitivity with recombinant protein or cell lysates.
• Cross-reactivity with mouse and rat is reported; however, always validate the antibody in the relevant species and sample system for your specific experimental conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw material sourcing partner, offering a comprehensive portfolio for assay development. This anti-SLC14A1 polyclonal antibody supports research into urea transport, renal physiology, and blood group antigen studies. Beyond single antibodies, CamelBio facilitates procurement of validated antibody pairs, bulk ancillary reagents, and customized sourcing solutions for rare or specialized targets, helping manufacturers streamline their pipeline from concept to clinic.