Anti-SLC14A1 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q13336

Core Product Specifications and Parameters

Biological Background: Urea Transporter 1 Function and Localization

• SLC14A1 encodes urea transporter 1 (UT-B), a member of the solute carrier family 14, also known as the Kidd (JK) blood group antigen.
• Mediates the transport of urea driven by a concentration gradient across the cell membrane of erythrocytes (PubMed:10514515, PubMed:7797558, PubMed:7989337). Related references: PMID:10514515, PMID:7797558, PMID:7989337
• Also mediates urea transport across the basolateral membrane of renal inner medullary collecting duct cells, playing a critical role in the urinary concentrating mechanism (By similarity).
• Facilitates water transport in erythrocytes (By similarity).
• Subcellular localization: predominantly expressed on the cell membrane, with basolateral polarization in renal epithelia.
• Detected in erythrocytes at the protein level (PubMed:23219802) and expressed in spleen erythroblasts and tumoral kidney (PubMed:7989337). Related references: PMID:23219802, PMID:7989337
• Post-translational modifications include glycosylation; the protein spans the membrane multiple times and exhibits alternative splicing, giving rise to several isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a synthetic peptide from the intracellular C-terminal region (amino acids 289–389). The antibody is expected to recognize an epitope that is not exposed on the cell surface, which may be relevant for applications requiring permeabilization.
• For Western blotting, a band around 43 kDa is anticipated; note that glycosylation may cause slight migration shifts. Optimize blocking and antibody dilution for your specific sample lysate.
• In IHC-P and IF/ICC, expect membrane staining. For intracellular epitope detection, permeabilization is required. Validate in positive control tissues such as kidney and erythroid lineage samples.
• For ELISA, consider using the recombinant extracellular domain or a peptide standard, and evaluate performance in sandwich pair setups.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material partner, offering diagnostic manufacturers and laboratories a streamlined sourcing experience from discovery to commercialization. This anti-SLC14A1 antibody supports research into urea transport mechanisms, renal physiology, and blood group antigen typing. Our comprehensive portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for niche targets, ensuring you have the critical materials needed for robust assay development.