Anti-SLC12A7 Polyclonal Antibody for WB, ELISA - Q9Y666

Core Product Specifications and Parameters

Biological Background: SLC12A7 Function and Localization

• Encodes solute carrier family 12 member 7, also known as K-Cl cotransporter 4 (KCC4), an electroneutral potassium-chloride cotransporter.
• Mediates electroneutral K+-Cl- cotransport activated by cell swelling, playing a role in cell volume regulation (PubMed:10913127). Related references: PMID:10913127
• Important for potassium recycling in the inner ear; may mediate K+ uptake into Deiters' cells, contributing to the survival of cochlear outer and inner hair cells and maintenance of the organ of Corti.
• In the kidney, may facilitate basolateral chloride extrusion and contribute to renal acidification (by similarity).
• Predominantly localized to the cell membrane as a multi-pass transmembrane protein.
• Detected in muscle, brain, lung, heart, and kidney tissues.
• Classified under keywords: alternative splicing, glycoprotein, phosphoprotein, disulfide bond, and ion transport, indicating complex regulation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-70), which may confer target specificity across species; consider sequence alignment to verify epitope conservation in mouse and rat.
• For Western blot, the predicted molecular weight is ~119 kDa; consider using appropriate gel percentage and transfer conditions for high-molecular-weight proteins.
• Validate reactivity in relevant tissue lysates (e.g., kidney, brain) and use appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-SLC12A7 polyclonal antibody supports studies on ion transport, renal physiology, and hearing research, and we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For rare or custom targets like SLC12A7, our sourcing network can help meet your specific raw-material needs.