Anti-SLC12A6 Polyclonal Antibody for IF/ICC, ELISA - Q9UHW9

Core Product Specifications and Parameters

Biological Background: SLC12A6 Function and Localization

• SLC12A6/KCC3 is an electroneutral potassium-chloride cotransporter that mediates K+ and Cl- efflux upon cell swelling, playing a critical role in cell volume homeostasis. Related references: PMID:10600773 PMID:11551954 PMID:16048901 PMID:27485015 PMID:33199848 PMID:34031912
• The protein localizes primarily to the basolateral cell membrane, consistent with its role in transepithelial ion transport.
• It is highly expressed in heart, brain, and kidney, with lower levels in skeletal muscle, placenta, lung, and pancreas; a testis-specific isoform has also been identified. Related references: PMID:21628467 PMID:10600773 PMID:16048901
• Mutations in SLC12A6 are associated with neurological disorders, including Charcot-Marie-Tooth disease type 2II and agenesis of the corpus callosum with peripheral neuropathy (ACCPN).
• The cotransporter is a glycoprotein and phosphoprotein, with multiple transmembrane domains and disulfide bonds contributing to its structure and regulation.
• The gene undergoes alternative splicing and alternative promoter usage, generating multiple isoforms with distinct tissue distributions.

Experimental Guidance and Technical Tips

• The immunogen (recombinant protein corresponding to amino acids 60–190 of human SLC12A6) targets the N-terminal region; antibody specificity should be confirmed in relevant human cell lines.
• For IF/ICC, optimize permeabilization conditions to detect membrane-bound SLC12A6, and include positive controls such as brain or kidney tissue sections.
• In ELISA applications, titrate the antibody to determine the optimal working concentration for antigen capture or detection; note that cross-reactivity is limited to human.
• Consider the impact of osmotic conditions on SLC12A6 expression and localization, as its function is swelling-activated.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing platform for IVD raw materials, providing diagnostic manufacturers and researchers with access to high-quality antibodies like this anti-SLC12A6 polyclonal antibody. Our portfolio includes validated antibody pairs, optimized monoclonal antibodies, and ancillary reagents to accelerate your ion channel and neurological disease research. From bulk orders to custom development, we support your workflow from concept to clinic.