Anti-SLC12A2 Polyclonal Antibody for WB, IHC-P, ELISA - P55011

Core Product Specifications and Parameters

Biological Background: SLC12A2 Function and Localization

• SLC12A2 (also known as NKCC1, BSC2, CCC1, BSC-2, KILQS, PPP1R141) is a cation-chloride cotransporter that mediates electroneutral transport of sodium, potassium, and chloride ions across the plasma membrane, playing a critical role in cell volume regulation and ionic homeostasis. Related references: PMID:16669787 PMID:32081947 PMID:32294086
• It is a member of the solute carrier family 12 (SLC12) of electroneutral cation-coupled chloride cotransporters, characterized by 12 transmembrane domains and intracellular N- and C-termini.
• SLC12A2 is localized to the basolateral membrane of epithelial cells and is widely expressed across many tissues, consistent with its housekeeping role in cell volume maintenance.
• The protein undergoes several post-translational modifications including phosphorylation and acetylation, which regulate its transport activity and membrane trafficking.
• Clinically, mutations in SLC12A2 are associated with autosomal recessive non-syndromic deafness and intellectual disability.
• SLC12A2 is a known target of the loop diuretic bumetanide, used in research and clinically to inhibit NKCC1 activity.

Experimental Guidance and Technical Tips

• The immunogen (aa 120-280) corresponds to an intracellular domain conserved across human, mouse, and rat, supporting cross-reactivity and targeting of total NKCC1.
• For Western blot detection of the 131 kDa protein, use a 6-8% SDS-PAGE gel and ensure efficient transfer via wet tank blotting with optimized conditions.
• In immunohistochemistry, confirm antibody specificity by using known positive (e.g., kidney, colon) and negative (e.g., NKCC1 knockout tissue) controls, and consider antigen retrieval methods.
• When employing ELISA, perform pairwise titration to determine optimal coating and detection concentrations; note that polyclonal nature may influence reproducibility.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic and research communities with comprehensive access to IVD raw materials. For ion transport and hearing loss studies, we offer validated antibody pairs against SLC12A2/NKCC1, bulk ancillary reagents, and sourcing of rare targets such as other SLC12 family members. Reach out to discuss your specific assay development and scale-up needs.