Anti-SLC12A1 Rabbit Polyclonal Antibody for WB, ELISA - Q13621

Core Product Specifications and Parameters

Biological Background: SLC12A1 Function and Localization

• SLC12A1 encodes the kidney-specific Na-K-2Cl cotransporter (NKCC2), a member of the solute carrier family 12, which mediates electroneutral symport of sodium, potassium, and chloride ions across the apical membrane of the thick ascending limb (TAL) of Henle’s loop. Related references: PMID:21321328
• NKCC2 is essential for transepithelial NaCl reabsorption in the TAL, driving urinary concentration and volume regulation; it serves as the primary target of loop diuretics such as bumetanide.
• The transporter can functionally substitute ammonium (NH4+) for potassium, facilitating apical NH4+ secretion in the medullary TAL, a process critical for renal ammonium homeostasis during metabolic acidosis.
• SLC12A1 expression is highly kidney-specific, with protein localization restricted to the apical plasma membrane of cells in the cortical and medullary thick ascending limbs.
• Post-translational modifications include N-linked glycosylation and phosphorylation, which regulate the transporter’s trafficking and activity in response to vasopressin and other hormonal signals.
• Mutations in SLC12A1 cause Bartter syndrome type 1, a salt-wasting renal disorder characterized by hypokalemic alkalosis, hypercalciuria, and polyuria, underscoring the critical role of NKCC2 in electrolyte balance.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to residues 50–150 of human SLC12A1. Verify epitope conservation if using non-human samples, although cross-reactivity with mouse and rat is reported.
• For Western blotting of NKCC2 (predicted MW ~121 kDa), consider using a low-percentage polyacrylamide gel (e.g., 6–8%) and prolonged transfer times to ensure efficient transfer of this large, glycosylated membrane protein.
• Include phosphatase inhibitors in lysis buffers and consider phosphatase treatment to resolve phosphorylation-dependent band shifts, as NKCC2 is regulated by phosphorylation in response to hormones.
• For ELISA applications, a competitive assay design with the immunizing peptide or a capture format using a recombinant extracellular domain fragment might be suitable; optimize coating buffer and blocking conditions to minimize nonspecific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop provider of IVD raw materials, offering validated antibodies, bulk reagents, and customized sourcing services. This anti-SLC12A1 polyclonal antibody supports research in renal ion transport and water homeostasis, and we can help you source complementary reagents for studying kidney function or developing diagnostic assays.