Anti-SLAMF7 Rabbit Polyclonal Antibody for WB, ELISA - Q9NQ25

Core Product Specifications and Parameters

Biological Background: SLAMF7 Function and Localization

• SLAMF7 is a self-ligand receptor of the SLAM family, also known as CD2 subset 1, CD2-like receptor-activating cytotoxic cells (CRACC), Membrane protein FOAP-12, Novel Ly9, Protein 19A, and CD319.
• Isoform 1 (canonical) mediates NK cell activation via a SH2D1A-independent ERK pathway and positively regulates NK cell functions downstream of SH2D1B, involving PLCG1, PLCG2, and PI3K. Related references: PMID:11698418 PMID:16339536
• In the absence of SH2D1B, isoform 1 inhibits NK cell function; it also acts inhibitory in T-cells.
• Isoform 3 does not mediate NK cell activation and is expressed at much lower levels than isoform 1.
• SLAMF7 may contribute to lymphocyte adhesion. Related references: PMID:11802771
• In LPS-activated monocytes, SLAMF7 negatively regulates production of pro-inflammatory cytokines. Related references: PMID:23695528
• SLAMF7 is a single-pass type I membrane protein with a signal peptide, immunoglobulin domain, disulfide bonds, and glycosylation. Keywords include Adaptive immunity, Innate immunity, Receptor, and Ig domain.
• Expressed in immune tissues and cells: spleen, lymph node, peripheral blood leukocytes, bone marrow, NK cells, activated B-cells, and monocytes.

Experimental Guidance and Technical Tips

• For Western blot, consider using whole cell lysates from NK or monocyte cell lines; the predicted molecular weight is 37 kDa, but glycosylation may cause a slightly higher band.
• The immunogen spans the extracellular region, suggesting the antibody may detect the receptor in native form; non-reducing conditions for ELISA may be considered.
• Cross-reactivity with mouse is listed; validate in mouse primary immune cells or tissues such as spleen.
• Due to alternative splicing, ensure isoform-specific detection if needed; validate with appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with one-stop access to IVD raw materials, technical services, and consulting. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. For SLAMF7 immunoassays, CamelBio can support your assay development with tailored antibody solutions and supply continuity.