Anti-SLAMF7 Polyclonal Antibody for WB and ELISA - Q9NQ25

Core Product Specifications and Parameters

Biological Background: SLAMF7 Function and Localization

• SLAMF7 (also known as CS1, CD319, CRACC, 19A) is a member of the signaling lymphocytic activation molecule (SLAM) family, functioning as a self-ligand receptor that modulates both innate and adaptive immune responses through homo- and heterotypic cell-cell interactions.
• Isoform 1 promotes natural killer (NK) cell activation via a SH2D1A-independent ERK-mediated pathway, while downstream signaling involves PLCG1, PLCG2, and PI3K in a SH2D1B-dependent manner. Related references: PMID:11698418 PMID:16339536
• In the absence of the adaptor protein SH2D1B, isoform 1 inhibits NK cell function, highlighting a regulatory duality; it can also exert inhibitory effects in T-cells.
• Isoform 3, expressed at lower levels, does not mediate NK cell activation.
• SLAMF7 may facilitate lymphocyte adhesion, potentially contributing to immune synapse formation. Related references: PMID:11802771
• In LPS-activated monocytes, SLAMF7 negatively regulates the production of pro-inflammatory cytokines. Related references: PMID:23695528
• The protein is localized to the cell membrane and is broadly expressed in immune tissues, including spleen, lymph node, peripheral blood leukocytes, bone marrow, and in specific cell types such as NK cells, activated B cells, and monocytes.
• As a transmembrane glycoprotein with a signal peptide, disulfide bonds, and an immunoglobulin domain, SLAMF7 is classified under keywords including adaptive immunity, innate immunity, and receptor activity.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 23-226 of the human SLAMF7 extracellular domain; for optimal performance, consider using denaturing and reducing conditions in Western blotting (WB) to detect the target band near the predicted molecular weight of 37 kDa, though glycosylation may cause a shift.
• For ELISA applications, the antibody may be used as a capture or detection reagent; due to its polyclonal nature, validate specificity using recombinant SLAMF7 protein and relevant negative controls.
• The antibody cross-reacts with mouse SLAMF7, enabling comparative studies across species; however, confirm performance in each sample matrix to account for potential expression level differences.
• Given the target’s role in NK cell and monocyte signaling, consider utilizing appropriate cell lines (e.g., NK-92) or primary cells to assess functional readouts; note that isoform-specific expression may influence experimental outcomes.

CamelBio: Your One-Stop Sourcing Bridge

• CamelBio supports diagnostic manufacturers, laboratories, and research institutes with comprehensive IVD raw material sourcing, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents.
• This anti-SLAMF7 polyclonal antibody serves as a reliable tool for immune function studies, particularly in NK cell and monocyte biology, and we can assist with custom reagent development and rare target sourcing to accelerate your project from concept to clinic.