Anti-SLAMF6 Rabbit Polyclonal Antibody for WB, ELISA - Q96DU3

Core Product Specifications and Parameters

Biological Background: SLAMF6 Function and Localization

• SLAMF6 is a member of the signaling lymphocytic activation molecule (SLAM) family, functioning as a self-ligand receptor that modulates immune cell activation and differentiation, influencing both innate and adaptive immunity. Its activities are regulated by small cytoplasmic adaptor proteins SH2D1A/SAP and SH2D1B/EAT-2.
• In NK cells, SLAMF6 triggers cytolytic activity, a process dependent on SH2D1B and involving VAV1 phosphorylation. In T cells, it promotes Th17 differentiation and IL-17 secretion via SH2D1A, facilitating RORC recruitment to the IL-17 promoter. These functions have been documented in key studies. Related references: PMID:11489943 PMID:16920955 PMID:22184727 PMID:22989874
• SLAMF6 localizes to the cell membrane, consistent with its role as a transmembrane receptor mediating cell–cell interactions.
• It is expressed on all resting and activated NK cells, T‑ and B‑lymphocytes. Surface expression is increased on T‑ cells of systemic lupus erythematosus (SLE) patients, highlighting its relevance in autoimmune conditions. Related references: PMID:11489943 PMID:22184727
• According to UniProt keywords, SLAMF6 undergoes alternative splicing, is a glycoprotein containing disulfide bonds, and is phosphorylated, indicating that post‑translational modifications may be critical for its function.
• The mature protein has a calculated molecular weight of approximately 37 kDa (UniProt: 37,345 Da), which is consistent with the supplier‑reported weight of 37 kDa.
• In the absence of adaptor proteins, SLAMF6 may act as a negative regulator of humoral immunity by inhibiting T‑cell:B‑cell adhesion and germinal center formation, and it is implicated in maintaining B‑cell tolerance. (By similarity)

Experimental Guidance and Technical Tips

• For Western blotting, consider that the predicted molecular weight is ~37 kDa. Due to glycosylation and other modifications, the observed band may appear at a higher molecular weight; comparing reducing and non‑reducing conditions can help confirm specificity.
• When performing ELISA, ensure the coating antigen matches the immunogen region (aa 24–215). Validate the antibody using relevant human lymphocyte samples or recombinant protein controls.
• As the antibody is raised against the extracellular domain, it is suitable for detecting surface‑expressed SLAMF6 in cell‑based assays under non‑denaturing conditions, but empirical validation in the specific sample system is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with consolidated access to IVD raw materials, technical services, and consulting—from concept to clinic. The SLAMF6 antibody offered here supports immunology research and assay development; CamelBio further supplies validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and can source rare‑target raw materials upon request. Let CamelBio streamline your supply chain with reliable, one‑stop procurement for your IVD projects.