Anti-SLA2 Rabbit Polyclonal Antibody for WB, ELISA - Q9H6Q3

Core Product Specifications and Parameters

Biological Background: SLA2 Function and Localization

• SLA2 (Src-like-adapter 2), also known as MARS and SLAP-2, is an adaptor protein containing SH2 and SH3 domains.
• It functions as a negative regulator of T-cell receptor (TCR) signaling, inhibiting the activation of nuclear factor of activated T-cells.
• SLA2 mediates signal attenuation by linking ZAP70 with CBL, leading to CBL-dependent degradation of signaling proteins.
• The protein localizes to the cytoplasm, cell membrane, and cytoplasmic vesicles.
• Predominantly expressed in immune tissues, with highest levels in peripheral blood leukocytes, spleen, thymus, and lymph nodes; also expressed in T-cells and monocytes.
• It is a myristoylated lipoprotein phosphoprotein, and undergoes alternative initiation and splicing.
• Synonyms include MARS (Modulator of Antigen Receptor Signaling) and SLAP-2, highlighting its role in immune regulation.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 1–160) covers the N-terminal region of SLA2, which may include SH2 and SH3 domains; consider this when analyzing band patterns in western blotting.
• For WB, the predicted protein weight is approximately 29 kDa; ensure appropriate gel percentage and transfer conditions for low molecular weight proteins.
• Validate antibody specificity in relevant human immune cell lysates (e.g., Jurkat T-cells, peripheral blood mononuclear cells) given the protein's tissue specificity.
• For ELISA applications, optimal coating conditions and antibody dilution should be determined empirically using recombinant SLA2 protein or peptide as standard.
• Cross-reactivity with other SLAP family members (e.g., SLAP1) has not been tested; consider using isoform-specific positive controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD manufacturers and research laboratories, providing access to high-quality raw materials such as SLA2 Rabbit Polyclonal Antibody for immunological and signaling research. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. From concept to clinic, CamelBio streamlines procurement to support your diagnostic and research workflows.