Anti-SKP2 Rabbit Polyclonal Antibody for Western Blot, IF/ICC, ELISA - Q13309

Core Product Specifications and Parameters

Biological Background: SKP2 Function and Localization

• SKP2 (S-phase kinase-associated protein 2), also known as p45, F-box protein Skp2, F-box/LRR-repeat protein 1, p45skp2, is a substrate recognition component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex.
• Core function in ubiquitin-mediated proteolysis: mediates ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Plays a critical role in G1/S transition by targeting phosphorylated CDKN1B/p27kip for degradation.
  Related references:
  PMID:9736735 PMID:11931757 PMID:12435635
• Recognizes a broad range of substrates, including ORC1, CDT1, RBL2, KMT2A/MLL1, CDK9, RAG2, NBN, FOXO1, UBP43, YTHDF2, and likely MYC, TOB1 and TAL1.
  Related references:
  PMID:11931757 PMID:12435635 PMID:12769844
• Promotes ubiquitination and destruction of CDH1 in a CK1-dependent manner, thereby regulating cell migration.
  Related references:
  PMID:22770219
• Following phosphorylation in response to DNA damage, mediates Lys-63-linked ubiquitination of NBN, promoting ATM recruitment to DNA damage sites and DNA repair via homologous recombination.
  Related references:
  PMID:22464731
• Exhibits antiviral activity through ubiquitin-mediated proteasomal degradation of hepatitis C virus non-structural protein 5A.
• Subcellular localization: cytoplasm and nucleus.
• Contains leucine-rich repeats and participates in the ubiquitin conjugation pathway; post-translational modifications include phosphorylation and acetylation.

Experimental Guidance and Technical Tips

• The immunogen, a synthetic peptide corresponding to amino acids 1–100 of human SKP2, was designed from the N‑terminal region; consider epitope accessibility in both denaturing (WB) and native (IF/ICC, ELISA) conditions.
• In Western blot, a band at approximately 48 kDa is anticipated (calculated MW ~47.8 kDa). Validate the detected band mass in your sample system.
• For immunofluorescence/ICC, optimize fixation and permeabilization protocols, as the polyclonal nature may produce background; include appropriate negative controls.
• Reported ELISA reactivity suggests utility in detecting native or recombinant SKP2; perform titration experiments to determine optimal antibody dilution.
• Cross‑reactivity with mouse and rat SKP2 is indicated; verify performance in species‑specific lysates or tissues prior to quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

As a trusted one‑stop partner for IVD raw material sourcing, CamelBio supports your cell cycle and ubiquitination research with validated reagents like this anti‑SKP2 polyclonal antibody. Beyond antibodies, we supply optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and offer rare‑target raw‑material sourcing to streamline your assay development from concept to clinic.