Anti-SKP2 Polyclonal Antibody for WB, IF/ICC, ELISA - Q13309

Core Product Specifications and Parameters

Biological Background: SKP2 Function and Localization

• SKP2 (S-phase kinase-associated protein 2), also known as p45, FBL1, FBXL1, is a substrate recognition component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex that mediates ubiquitination and proteasomal degradation of target proteins involved in cell cycle progression, signal transduction, and transcription. Related references: PMID:9736735, PMID:11931757, PMID:12435635
• SKP2 specifically recognizes phosphorylated CDKN1B/p27kip, promoting its degradation to regulate G1/S transition; this process also requires the cofactor CKS1.
• It targets a wide array of substrates, including ORC1, CDT1, RBL2, KMT2A/MLL1, CDK9, RAG2, NBN, FOXO1, UBP43, YTHDF2, and likely MYC, TOB1, and TAL1. Degradation of TAL1 requires cooperation with the E3 ligase STUB1. Related references: PMID:11931757, PMID:12435635, PMID:12769844, PMID:17962192
• In complex with cyclin E (CCNE1 or CCNE2) and CDK2, SKP2 recognizes and promotes ubiquitination of CDKN1A/p21. Related references: PMID:9736735, PMID:16262255
• Upon DNA damage, phosphorylated SKP2 mediates Lys-63-linked ubiquitination of NBN, facilitating ATM recruitment and promoting homologous recombination repair. Related references: PMID:22464731
• SKP2 also promotes CK1-dependent ubiquitination and degradation of CDH1 to regulate cell migration, and exhibits antiviral activity by targeting hepatitis C virus NS5A for proteasomal degradation. Related references: PMID:22770219
• The protein localizes to both the cytoplasm and nucleus and is subject to post-translational modifications including acetylation and phosphorylation; it contains leucine-rich repeats characteristic of F-box proteins.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 100–200 of human SKP2; confirm that this region is sufficient for detecting the target in your specific application and sample type.
• For Western blot, consider a starting dilution range of 1:500–1:2000, and optimize based on expression levels in your model. A band near 48 kDa is expected.
• In immunofluorescence/ICC, include appropriate fixation and permeabilization steps to detect SKP2 in both cytoplasmic and nuclear compartments.
• For ELISA, evaluate compatibility with your coating buffer and detection system, and consider using this antibody as either capture or detection reagent after pairing validation.
• This antibody is raised against human SKP2; cross-reactivity with other species has not been determined. Validate specificity if used with non-human samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with integrated access to IVD raw materials, streamlining the journey from concept to clinic. This anti-SKP2 polyclonal antibody supports research in cell cycle regulation, ubiquitin-proteasome pathways, and antiviral mechanisms. Beyond this product, CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and specialized sourcing for rare-target raw materials to accelerate your assay development and manufacturing needs.