Anti-SKP2 Monoclonal Antibody for WB, IHC-P, ELISA - Q13309

Core Product Specifications and Parameters

Biological Background: SKP2 Function and Localization

• SKP2 (S-phase kinase-associated protein 2) is an F-box protein that serves as the substrate recognition subunit of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex. It targets key cell cycle regulators, such as CDKN1B/p27 and CDKN1A, for ubiquitination and proteasomal degradation, thereby promoting G1/S transition (PubMed:9736735, PubMed:11931757, PubMed:12435635). Related references: PMID:9736735, PMID:11931757, PMID:12435635
• Beyond cell cycle control, SKP2 mediates ubiquitination of diverse substrates including CDT1, ORC1, FOXO1, MYC, and CDH1, influencing DNA replication, apoptosis, transcription, and cell migration (PubMed:12769844, PubMed:22770219). Related references: PMID:12769844, PMID:22770219
• In response to DNA damage, phosphorylated SKP2 catalyzes Lys-63-linked ubiquitination of NBN, facilitating ATM recruitment and homologous recombination repair (PubMed:22464731). Related references: PMID:22464731
• SKP2 also possesses antiviral activity by targeting hepatitis C virus non-structural protein 5A for proteasomal degradation, linking it to innate immunity.
• Localized to both the nucleus and cytoplasm, SKP2 undergoes post-translational modifications including phosphorylation and acetylation, which modulate its stability and function.
• SKP2 is a member of the F-box/LRR-repeat protein family (FBXL1) and is frequently overexpressed in various cancers, making it a potential diagnostic and therapeutic target.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic peptide from the N-terminal region (aa 1-100) of human SKP2. For Western blotting, a band at approximately 48 kDa is expected in human cell lysates; consider using a reducing gel and validate with a positive control such as HeLa or 293T cells.
• For immunohistochemistry (paraffin-embedded tissue), antigen retrieval methods and antibody dilution require optimization; include appropriate tissue controls known to express SKP2 (e.g., tonsil, breast carcinoma).
• In ELISA applications, use the recombinant antibody as a capture or detection reagent in combination with validated antigen or matched antibody pairs; confirm performance with recombinant SKP2 protein.
• Cross-reactivity with other species has not been reported; this product is intended for human samples. Researchers should independently verify specificity in their model system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a complete IVD raw-material partner, offering everything from validated antibody pairs to bulk ancillary reagents, supporting your diagnostic development from concept to clinic. For SKP2-focused projects—whether you need monoclonal antibodies for cell cycle assays, ubiquitination studies, or cancer biomarker panels—CamelBio provides optimized sourcing and technical consultancy. Our global supply network also handles rare targets and custom production, ensuring consistent quality and scalability for IVD manufacturers and research laboratories.