Anti-SKP1 Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - P63208

Core Product Specifications and Parameters

Biological Background: SKP1 Function and Localization

• SKP1 (S-phase kinase-associated protein 1), also known as p19A, OCP-2, and TCEB1L, is an essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex.
• It functions as an adapter linking F-box proteins to CUL1, enabling substrate recognition and ubiquitination.
• The SCF(SKP2) complex directs ubiquitination of phosphorylated CDKN1B/p27kip, regulating G1/S transition in the cell cycle.
• SCF(BTRC) and SCF(FBXW11) direct ubiquitination of β-catenin (CTNNB1) and participate in Wnt signaling.
• SCF(FBXW11) directs ubiquitination of phosphorylated NFKBIA (IκBα), leading to NF-κB activation.
• SCF(BTRC) also directs ubiquitination of multiple substrates including NFKBIB, NFKBIE, ATF4, SMAD3, SMAD4, CDC25A, FBXO5, and CEP68 (PubMed:25704143). Related references: PMID:25704143
• SCF(FBXL3) and SCF(FBXL21) direct ubiquitination of circadian clock proteins CRY1 and CRY2.
• SKP1 is a phosphoprotein with alternative splicing, and it interacts with viral proteins (host-virus interaction).
• SKP1 acts as a core component of the Cul7-RING(FBXW8) ubiquitin ligase complex (PubMed:35982156) and the Cul1-RING(FBXL4) complex that mediates degradation of BNIP3/BNIP3L (PubMed:36896912). Related references: PMID:35982156 PMID:36896912

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 64–163 of human SKP1; thus, the antibody is predicted to recognize an epitope within this central region. For species cross-reactivity, validate in the relevant sample system.
• For Western blot (WB), expect a band at approximately 19 kDa, though post-translational modifications may cause slight shifts. Optimize lysate preparation and antibody dilution for best results.
• For immunofluorescence/immunocytochemistry (IF/ICC), choose fixation and permeabilization conditions appropriate for nuclear and cytoplasmic targets, as SKP1 localizes throughout the cell.
• In ELISA applications, ensure the peptide or protein coating matches the immunogen region to achieve optimal detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers one-stop access to IVD raw materials, including validated antibodies and ancillary reagents. This anti-SKP1 monoclonal antibody supports studies on ubiquitin-proteasome pathways, cell cycle regulation, and signal transduction. For bulk orders, custom conjugations, or sourcing rare target materials, CamelBio’s supply chain expertise streamlines your development from concept to clinic.