Anti-SKIV2L2 Polyclonal Antibody for WB, IHC-P, IP, ELISA - P42285

Core Product Specifications and Parameters

Biological Background: SKIV2L2 (MTR4) Function and Localization

• SKIV2L2, also known as MTR4, DOB1, or KIAA0052, is an ATP-dependent RNA helicase encoded by the MTREX gene in humans. It catalyzes ATP-dependent unwinding of RNA duplexes with a single-stranded 3' RNA extension.
  Related references: PMID:27871484 PMID:29844170 PMID:29906447
• SKIV2L2 serves as a central subunit of the nuclear exosome targeting (NEXT), poly(A) tail exosome targeting (PAXT), and TRAMP-like complexes, directing subsets of non-coding and aberrant transcripts for exosomal degradation.
  Related references: PMID:21855801 PMID:27871484 PMID:29844170
• It is involved in the 3'-processing of 7S pre-rRNA to mature 5.8S rRNA within the RNA exosome complex.
  Related references: PMID:17412707 PMID:29107693
• SKIV2L2 may participate in pre-mRNA splicing and is a component of the spliceosome.
• It can unwind DNA:RNA heteroduplexes and displace DNA strands while translocating on RNA, facilitating the degradation of structured RNA substrates.
  Related references: PMID:29844170 PMID:29906447
• The protein is localized to the nucleoplasm, nucleolus, and nuclear speckles.
• Post-translational modifications include acetylation and phosphorylation, with an isopeptide bond formation.
• SKIV2L2 also plays a role in the DNA damage response.
  Related references: PMID:29902117

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-304 of human SKIV2L2 (N-terminal region), which may aid in detecting the full-length protein in Western blotting.
• For Western blot (WB), the predicted molecular weight is approximately 118 kDa; ensure appropriate gel percentage and transfer conditions.
• In immunohistochemistry (IHC-P), antigen retrieval and antibody titration are recommended to optimize staining specificity.
• For immunoprecipitation (IP), use appropriate controls, such as non-immune IgG, to confirm specific interactions.
• The antibody cross-reacts with human, mouse, and rat SKIV2L2, making it suitable for studies across these species, but validation in the target species' tissue or cell type is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For SKIV2L2/MTR4-related research in RNA biology and disease mechanisms, we offer a reliable supply of validated polyclonal antibodies, as well as monoclonal antibody development and bulk ancillary reagents to support your assay development and production needs. Whether you require standard catalog products or custom-sourced rare-target reagents, our integrated supply chain facilitates seamless progression from early-stage R&D to clinical application.