Anti-SKIL Rabbit Polyclonal Antibody for WB, ELISA - P12757

Core Product Specifications and Parameters

Biological Background: SKIL Function and Localization

• SKIL (Ski-like protein), also known as SnoN, SnoA, and SnoI, is encoded by the SKIL gene in humans. It is a member of the Ski family of transcriptional regulators.
• The protein is implicated in the regulation of cell division or differentiation in response to extracellular signals, potentially acting as an oncogene.
• Alternative splicing generates multiple isoforms: SNON and SNOA are widely expressed, with highest levels in skeletal muscle, placenta, and lung; SNOI expression is restricted to skeletal muscle.
• SKIL undergoes post-translational modifications including phosphorylation and ubiquitination, and contains a coiled-coil domain that facilitates protein-protein interactions.

Experimental Guidance and Technical Tips

• The immunogen used is a synthetic peptide corresponding to amino acids 350-450 of human SKIL (NP_005405.2). For Western blot, consider using whole cell lysates from tissues with high SKIL expression such as skeletal muscle, placenta, or lung. The predicted molecular weight is approximately 77 kDa, but isoforms may run at different sizes.
• For ELISA, the antibody may be employed for detecting recombinant SKIL or peptide fragments. Validate the antibody in your specific assay conditions, including optimal coating and detection protocols.
• Cross-reactivity with mouse and rat SKIL has been reported, but sequence alignment in the immunogen region is recommended to confirm potential cross-reactivity in other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive IVD raw material solutions, providing researchers and manufacturers with validated antibodies, ancillary reagents, and bulk sourcing capabilities. This anti-SKIL polyclonal antibody is a reliable tool for investigating cell division and differentiation processes, particularly in oncology and developmental biology research. For complete assay development, CamelBio also supplies paired antibodies, recombinant proteins, and technical consultation to support your projects from concept to clinic.